For H&E staining, the left lungs of mice were fixed with 4% PFA, dehydrated, embedded in paraffin, and cut into 5‐µm‐thick sections. The tissue sections were deparaffinized and stained with H&E using a Leica ST5020‐CV5030 Multistainer‐Coverslipper (Germany).
Tissue microarray slides of NSCLC patients were obtained from Superchip Company (China). After deparaffinization and antigen retrieval, IHC was performed using the IHC Detection Kit (#ab64264, Abcam, UK) according to the manufacturer's instructions. Nuclei were stained with hematoxylin by a Leica ST5020‐CV5030 Multistainer‐Coverslipper (Germany). All slides were scanned with a NanoZoomer S60 Digital slide scanner (#C13210‐01, HAMAMATSU, Japan) to acquire whole‐view images. The IHC scores were composed of percentage scores and intensity scores as previously described.[87 ]
Tissue microarray slides of NSCLC patients were obtained from Superchip Company (China). After deparaffinization and antigen retrieval, IHC was performed using the IHC Detection Kit (#ab64264, Abcam, UK) according to the manufacturer's instructions. Nuclei were stained with hematoxylin by a Leica ST5020‐CV5030 Multistainer‐Coverslipper (Germany). All slides were scanned with a NanoZoomer S60 Digital slide scanner (#C13210‐01, HAMAMATSU, Japan) to acquire whole‐view images. The IHC scores were composed of percentage scores and intensity scores as previously described.[