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Annexin 5 binding solution

Manufactured by BD
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Annexin V binding solution is a product designed for cell biology research. It is a reagent used to detect and quantify apoptosis, a programmed cell death process, in cell samples. The solution contains Annexin V, a protein that binds to phosphatidylserine, a molecule exposed on the surface of apoptotic cells.

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Lab products found in correlation

Tryple express enzyme, by Thermo Fisher Scientific (2 mentions) Matlab, by MathWorks (2 mentions) Pi rnase staining solution, by BD (1 mentions)

Close spelling variants of this product

Annexin V-FITC binding solution 1 × Annexin V-binding buffer solution Annexin V binding Annexin V solution Binding solution Annexin V

3 protocols using annexin 5 binding solution

1

Annexin V Apoptosis Assay by Flow Cytometry

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After treatment, floating cells in growth medium were combined with adherent cells that were detached using TrypLE™ Express Enzyme (ThermoFisher Scientific, Waltham, MA, catalog no. 12604013). Cells were then washed 3x in cold PBS, resuspended in 1x Annexin V binding solution (BD Biosciences, San Jose, CA, catalog no. 556454) and stained with Annexin V conjugated with FITC (BD Biosciences, San Jose, CA, catalog no. 556419). Apoptotic cells were quantified by flow cytometry. Results were imported into Matlab (Mathworks, Natick,MA) and processed.
Feng D., Qin B., Pal K., Sun L., Dutta S., Dong H., Liu X., Mukhopadhyay D., Huang S, & Sinicrope F.A. (2019). BRAFV600E-induced, tumor intrinsic PD-L1 can regulate chemotherapy-induced apoptosis in human colon cancer cells and in tumor xenografts. Oncogene, 38(41), 6752-6766.
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2

Apoptosis Evaluation by Flow Cytometry

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After 96 h of infection, the cells were trypsinized, centrifuged at 1000 r/min for 5 min, and resuspended in annexin V binding solution (BD company, USA) to prepare a single-cell suspension. PI/RNase staining solution (BD, USA) was added to the suspension, gently mixed, and incubated in the dark at 4 °C. Flow cytometry analysis was performed within 30 min.
Han Y., Maimaiti N., Sun Y, & Yao J. (2024). Knockout of KDM3A in MDA-MB-231 breast cancer cells inhibits tumor malignancy and promotes apoptosis. Journal of Molecular Histology, 55(1), 139-148.
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3

Annexin V Apoptosis Assay by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols
After treatment, floating cells in growth medium were combined with adherent cells that were detached using TrypLE™ Express Enzyme (ThermoFisher Scientific, Waltham, MA, catalog no. 12604013). Cells were then washed 3x in cold PBS, resuspended in 1x Annexin V binding solution (BD Biosciences, San Jose, CA, catalog no. 556454) and stained with Annexin V conjugated with FITC (BD Biosciences, San Jose, CA, catalog no. 556419). Apoptotic cells were quantified by flow cytometry. Results were imported into Matlab (Mathworks, Natick,MA) and processed.
Feng D., Qin B., Pal K., Sun L., Dutta S., Dong H., Liu X., Mukhopadhyay D., Huang S, & Sinicrope F.A. (2019). BRAFV600E-induced, tumor intrinsic PD-L1 can regulate chemotherapy-induced apoptosis in human colon cancer cells and in tumor xenografts. Oncogene, 38(41), 6752-6766.
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