Fluorescein isothiocyanate annexin 5 apoptosis detection kit 2

Manufactured by BD

Sourced in United States

The Fluorescein Isothiocyanate Annexin V Apoptosis Detection kit II is a laboratory reagent used to detect and quantify apoptosis (programmed cell death) in cell samples. The kit utilizes Annexin V, a protein that binds to phosphatidylserine, and the fluorescent dye Fluorescein Isothiocyanate (FITC) to label cells undergoing apoptosis. This allows for the identification and analysis of apoptotic cells using flow cytometry or fluorescence microscopy.

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Lab products found in correlation

Version 10, by FlowJo (1 mentions) Canto 2, by BD (1 mentions) Gallios flow cytometer, by Beckman Coulter (1 mentions)

Close spelling variants of this product

Annexin V-fluorescein isothiocyanate (FITC) Apoptosis Detection Kit II Annexin V-fluorescein isothiocyanate apoptosis detection kit Annexin V-fluorescein-5-isothiocyanate apoptosis detection kit Annexin V-fluorescein isothiocyanate apoptosis kit Annexin V Apoptosis Detection Kit II Annexin V-fluorescein isothiocyanate kit Annexin V Apoptosis Detection Kit Annexin V-fluorescein isothiocyanate Annexin V Apoptosis Kit Annexin V detection kit

2 protocols using fluorescein isothiocyanate annexin 5 apoptosis detection kit 2

Quantifying Transfected KC Apoptosis

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Transfected primary KCs were cultured for 48 hours. Cells were collected and then detected with a Fluorescein Isothiocyanate Annexin V Apoptosis Detection kit II (BD Pharmingen; BD Biosciences, Franklin Lakes, NJ, USA) according to the manufacturer’s protocol. The percentage of apoptotic cells were measured by using a flow cytometer (BD Canto II; BD Biosciences). The data were analyzed by FlowJo version 10 (FlowJo LLC, Ashland, OR, USA).

Zeng J., Zhang Y., Zhang H., Zhang Y., Gao L., Tong X., Xie Y., Hu Q., Chen C., Ding S, & Lu J. (2021). RPL22 Overexpression Promotes Psoriasis-Like Lesion by Inducing Keratinocytes Abnormal Biological Behavior. Frontiers in Immunology, 12, 699900.

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Quantification of Apoptosis in Monocytes

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To quantitate apoptosis, we used the fluorescein isothiocyanate Annexin V Apoptosis Detection Kit II (BD Biosciences, San Jose, Calif). Monocytes were cultured with GM-CSF, IL-1b, or LPS alone or in combination with monosodium urate (MSU) crystals and ATP over 24 hours. As a positive control, monocytes were cultured with 10 mmol/L camptothecin for 4 hours. Approximately 1 3 10 6 cells/mL were stained with Annexin V conjugated with fluorescein isothiocyanate and propidium iodide and processed on a Gallios Flow Cytometer (Beckman Coulter, Pasadena, Calif) and subsequently analyzed by using FlowJo software (Tree Star, Ashland, Ore).

Vento-Tormo R., Álvarez-Errico D., Garcia-Gomez A., Hernández-Rodríguez J., Buján S., Basagaña M., Méndez M., Yagüe J., Juan M., Aróstegui J.I, & Ballestar E. (2017). DNA demethylation of inflammasome-associated genes is enhanced in patients with cryopyrin-associated periodic syndromes. The Journal of allergy and clinical immunology, 139(1).

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