Reagents for solid phase peptide synthesis were purchased from Apptec (Louisville, KY) except 5-azido pentanoic acid which was purchased from Bachem (Torrance, CA). All other chemicals were purchased from Sigma-Aldrich (St. Louis, MO). Cell culture reagents were purchased from Cellgro/Mediatech (Fisher Scientific, Pittsburgh PA). The pCMV-Luc2 plasmid was isolated using the Qiagen Plasmid Giga Kit (Hilden, Germany). The bicinchoninic acid (BCA) protein quantification assay kit was purchased from Thermo Fischer Scientific (Waltham, MA) while the luciferase expression quantification kit and MTS assay were obtained from Promega (Madison, WI).
5 azido pentanoic acid
Manufactured by Bachem
Sourced in Switzerland
5-azido pentanoic acid is a chemical compound used in various laboratory applications. It has a chemical formula of C5H9N3O2 and a molecular weight of 159.15 g/mol. The primary function of this compound is to serve as a versatile building block in organic synthesis and chemical research.
Automatically generated - may contain errors
Lab products found in correlation
Luciferase expression quantification kit, by Promega (1 mentions)
Bicinchoninic acid bca protein quantification assay kit, by Thermo Fisher Scientific (1 mentions)
Mts assay, by Promega (1 mentions)
Plasmid giga kit, by Qiagen (1 mentions)
Methacryloyl chloride, by Merck Group (1 mentions)
N n diisopropylcarbodiimide, by Merck Group (1 mentions)
Atto 488 nhs, by ATTO-TEC (1 mentions)
Dichloromethane, by Merck Group (1 mentions)
Triisopropylsilane, by Merck Group (1 mentions)
Atto 488 nh2, by ATTO-TEC (1 mentions)
2 protocols using 5 azido pentanoic acid
1
Peptide Synthesis and Cell Assays
2
Synthesis of Clickable Fluorescent Monomers
Check if the same lab product or an alternative is used in the 5 most similar protocols
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1-Aminopropan-2-ol, 2,2’-azobis-(isobutyronitrile) (AIBN), dichloromethane (DCM), N, N′-diisopropylcarbodiimide (DIC), N, N’-dimethylacetamide (DMA), N, N′-dimethylformamide (DMF), ethyldiisopropylamine (DIPEA), hydrazine monohydrate, methacryloyl chloride, triisopropylsilane (TIPS) and all other reagents and solvents were purchased from Sigma-Aldrich (Sigma-Aldrich, Prague, Czech Republic). Chain-transfer agents and initiators were purchased from FUJIFILM Wako Pure Chemical Corporation (FUJIFILM Wako Pure Chemical Corporation, Neuss, Germany). TentaGel Rink amide resin, ethyl cyano(hydroxyimino)acetate (Oxyma), (benzotriazol-1-yloxy)-trispyrrolidinophosphoniumhexafluorophosphate (PyBOP), trifluoroacetic acid (TFA), 9-fluorenylmethyloxycarbonyl (Fmoc)-amino acid derivatives were purchased from Iris Biotech GmbH (Iris Biotech GmbH, Marktredwitz, Germany). 5-Azidopentanoic acid was obtained from Bachem (Bachem, Bubendorf, Switzerland) and amino-1-(11,12-didehydrodibenzo[b,f]azocin-5(6H)-yl)propan-1-one (DBCO-NH2) was purchased from Click Chemistry Tools (Click Chemistry Tools, Scottsdale, AZ, USA). ATTO-488-NHS and ATTO-488-NH2 were purchased from ATTO-TEC GmbH (ATTO-TEC GmbH, Siegen, Germany).
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