DNA was extracted from single colonies with a TIANamp Bacteria Kit (Tiangen) and tested by PCR for the wild‐type target gene and mutated alleles using the primers listed in Table S3 . The resulting PCR products were analysed by agarose gel electrophoresis and sequenced to confirm the presence of the desired mutation (RuiBiotech).
Tianamp bacteria kit
Manufactured by Tiangen Biotech
The TIANamp bacteria kit is a product designed for the rapid and efficient extraction of high-quality genomic DNA from various bacterial strains. It utilizes a spin column-based method to isolate DNA, ensuring a convenient and reliable workflow.
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Lab products found in correlation
3 protocols using tianamp bacteria kit
1
Bacterial DNA Extraction and Mutation Analysis
2
E. coli Chromosomal and Plasmid DNA Isolation
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The chromosomal DNA isolation from E. coli strain K-12 substrain MG1655 was achieved using the TIANamp bacteria kit (Tiangen, Beijing, PRC). Subsequently, plasmids were isolated using the TIANprep minikit, and DNA was purified using the TIANgel midi kit. All the above procedures were performed following the specified protocols from the manufacturer.
3
E. coli Chromosomal and Plasmid DNA Isolation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The chromosomal DNA isolation from E. coli strain K-12 substrain MG1655 was achieved using the TIANamp bacteria kit (Tiangen, Beijing, PRC). Subsequently, plasmids were isolated using the TIANprep minikit, and DNA was purified using the TIANgel midi kit. All the above procedures were performed following the specified protocols from the manufacturer.
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