Water-soluble CdSe core NCs were obtained using mercaptosuccinic acid as a surface-modifying agent. Briefly, 20–30 mg of NCs was dissolved in chloroform, after which 3 mL of dimercaptosuccinic acid (Sigma, dissolved in 150 mM PBS, pH 7.3) was added to 6 mL of NCs in chloroform. Water containing water-soluble CdSe NCs was carefully decanted and subjected to ultrafiltration to remove free (nonreacted) dimercaptosuccinic acid. The ultrafiltration was carried out using a concentrator (Vivaspin-6/20, Sartorius AG, Gottingen, Germany).
Vivaspin 6 20
Manufactured by Sartorius
Sourced in Germany
The Vivaspin-6/20 is a centrifugal concentrator used for the rapid concentration and desalting of macromolecular solutions. It features a vertically oriented membrane design that allows for efficient volume reduction and sample recovery.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using vivaspin 6 20
1
Synthesis of Water-Soluble CdSe Nanocrystals
2
Ultrafiltration for Sample Concentration
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Ultrafiltration units with various cutoffs (Vivaspin 6-20, Sartorius, Germany) were first rinsed by centrifuging pure water through the membrane. The medium to be analyzed was then centrifuged at 6000 × g until the supernatant reached a tenth of its initial volume, and both the concentrated supernatant and filtrate fractions were collected in plastic tubes and used for experiments.
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