Human insulin levels in mouse blood were measured based on a previously reported method.55 (link) Mouse blood samples were collected into heparin-coated blood collection tubes (Sarstedt Microvette; CB 300 LH). Samples were centrifuged at 3000 g for 3 min, and plasma samples were collected into PCR tubes. Human insulin level measurements were performed with an Ultrasensitive Human Insulin ELISA kit (Mercodia) based on the manufacturer’s protocol. Standard curves were fitted with a linear model.
Ultrasensitive human insulin elisa kit
Manufactured by Mercodia
The Ultrasensitive Human Insulin ELISA kit is a laboratory equipment designed to measure insulin levels in human samples. It utilizes the enzyme-linked immunosorbent assay (ELISA) technique to quantify insulin with high sensitivity.
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Lab products found in correlation
2 protocols using ultrasensitive human insulin elisa kit
1
Measuring Mouse Plasma Human Insulin
2
Insulin Secretion Measurement Protocol
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INS release was measured by incubating the cells in Functionality/Viability Medium CMRL1066 (Mediatech). The cells were washed three times in PBS and incubated in the solution (Functionality/Viability Medium CMRL1066) with 2.8 mM D-glucose six times for 20 min each (total 2 h) to wash them. The cells were then incubated in the solution with 2.8 mM D-glucose for 2 h and then in the solution with 20 mM D-glucose for 2 h. The INS levels in the culture supernatants were measured using an Ultrasensitive Human Insulin ELISA kit (Mercodia).
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