Secreted cytokines were accumulated in cell growth medium for 2d. ELISA for IFN-alpha (eBioscience), IFN-gamma (OptEIA Human IFN gamma ELISA Set, BD Biosciences), IP-10 (interferon-inducible protein 10, CXCL10), IL-8, and MCP-1 (monocyte chemoattractant protein-1, CCL2) (all from R&D Systems) were performed in duplicates according to the manufacturer’s instructions. Optical density was measured at 450 nm; the data were analyzed with the MicroWin software (Berthold Technologies). Alternatively, cytokine levels in the cell growth medium were determined in duplicates by a bead-based multiplex immunoassay (FlowCytomix from eBioscience) according to the manufacturer’s instructions. Data were acquired on a FACSCalibur and evaluated with the FlowCytomixPro software (eBioscience).
Opteia human ifn gamma elisa set
Manufactured by BD
The OptEIA Human IFN gamma ELISA Set is a laboratory equipment product that is used for the quantitative measurement of human interferon gamma (IFN-gamma) levels in various sample types. It is a sandwich enzyme-linked immunosorbent assay (ELISA) kit designed to provide accurate and reliable results.
Automatically generated - may contain errors
Lab products found in correlation
Microwin software, by Berthold Technologies (2 mentions)
Facscalibur, by Thermo Fisher Scientific (1 mentions)
Flowcytomix, by Thermo Fisher Scientific (1 mentions)
Flowcytomix pro software, by Thermo Fisher Scientific (1 mentions)
Ficoll, by Harvard Bioscience (1 mentions)
Anti pd 1, by Miltenyi Biotec (1 mentions)
Rpmi 1640, by Lonza (1 mentions)
Ultraglutamine, by Lonza (1 mentions)
Penicillin, by Lonza (1 mentions)
Streptomycin, by Lonza (1 mentions)
Fetal calf serum (fcs), by Lonza (1 mentions)
2 protocols using opteia human ifn gamma elisa set
1
Cytokine Profiling by ELISA and Multiplex Assay
2
Stimulation of PBMC for IFN-gamma production
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Buffy coats from anonymized healthy donors were obtained from the “DRK-Blutspendedienst – Ost”. Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation using Ficoll (Biochrom). Cells were cultured in complete medium (RPMI1640 [Lonza] with 2 mM UltraGlutamine [Lonza] supplemented with 10% [v/v] fetal calf serum [Linaris], 100 U/ml Penicillin and 100 µg/ml Streptomycin [Lonza]) in flat-bottom plates (PBMC, 6 million cells/ml). Cells were stimulated with 1 µg/ml extended CEF (cytomegalo virus, Epstein-Barr virus, influenza virus) peptide pool (JPT Peptide Technologies GmbH), 3 µM lefitolimod and 10 µg/ml anti-PD-1 (Miltenyi Biotec) for 2 days. IFN-gamma in the culture supernatant was determined by ELISA (OptEIA Human IFN gamma ELISA Set, BD Biosciences) and was performed in duplicates according to the manufacturer’s instructions. Optical density was measured at 450 nm; the data were analyzed with the MicroWin software (Berthold Technologies).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!