Mouse anti βii spectrin

Manufactured by BD

Mouse anti-βII-spectrin is a laboratory reagent used for the detection and identification of βII-spectrin, a structural protein found in the cytoskeleton of various cell types. It is commonly used in research applications that require the specific recognition and visualization of βII-spectrin in biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Rhodamine phalloidin, by Thermo Fisher Scientific (1 mentions) Mouse monoclonal anti acetylated tubulin clone 6 11b 1, by Merck Group (1 mentions) Rabbit anti tom20, by Santa Cruz Biotechnology (1 mentions) Rat anti tyrosinated tubulin, by Abcam (1 mentions) Mouse anti α tubulin, by Merck Group (1 mentions)

Spelling variants of this product

Mouse anti (2 citations)

3 protocols using mouse anti βii spectrin

Immunofluorescence Imaging of Microtubule Cytoskeleton

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies directed against βIII tubulin (Tuj-1, 1:1,000 for immunofluorescence) were obtained from Millipore. Cucurbitacin-E (5 nM, SML0577), Paclitaxel (Taxol, 10 µM), mouse monoclonal anti-acetylated tubulin (Clone 6-11B-1) and anti-alpha-tubulin (clone DM1A) were from Sigma. Phalloidin-ATTO647N (used for STED) was from ATTO-TEC and phalloidin-rhodamine (used for widefield) from Thermo Fisher. Rat anti-tyr-tubulin antibody was from Abcam (clone YL1/2, cat#ab6160). Rabbit anti-glu-tubulin was a kind gift from Dr Gregg G. Gundersen (Columbia University Medical Center, US) and was used previously^{35 (link)}. Mouse anti-βII-spectrin was from BD Biosciences (US, cat#612563).

Unsain N., Bordenave M.D., Martinez G.F., Jalil S., von Bilderling C., Barabas F.M., Masullo L.A., Johnstone A.D., Barker P.A., Bisbal M., Stefani F.D, & Cáceres A.O. (2018). Remodeling of the Actin/Spectrin Membrane-associated Periodic Skeleton, Growth Cone Collapse and F-Actin Decrease during Axonal Degeneration. Scientific Reports, 8, 3007.

+ Open protocol

+ Expand

Immunofluorescence Labeling of Mitochondria and Synaptic Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were fixed in 4% PFA in PBS for ~20 min. For immunofluorescence labeling, cells were first blocked with a solution of 3% bovine serum albumin and 0.1% saponin in PBS, and then stained with corresponding primary and secondary antibodies in the same blocking solution. Primary antibodies used were mouse anti-βII spectrin (BD, 612562; 1:50), rabbit anti-TOM20 (Santa Cruz, sc-11415; 1:200), and mouse anti-mitochondrial Complex II (Invitrogen, 459230; 1:400). For STORM imaging of synaptosomes, an Alexa Fluor 647-conjugated secondary antibody was used to label βII spectrin. For STORM imaging of mitochondria, Alexa Fluor 647-conjugated and Alexa Fluor 555-conjugated secondary antibodies (at 5μg/mL) were used to label mitochondria Complex II and TOM20, respectively.

Polyzos A.A., Lee D.Y., Datta R., Hauser M., Budworth H., Holt A., Mihalik S., Goldschmidt P., Frankel K., Trego K., Bennett M.J., Vockley J., Xu K., Gratton E, & McMurray C.T. (2019). Metabolic Reprogramming in Astrocytes Distinguishes Region-Specific Neuronal Susceptibility in Huntington Mice. Cell metabolism, 29(6), 1258-1273.e11.

+ Open protocol

+ Expand

Multicolor Immunofluorescence Staining

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mouse anti-βII-spectrin was from BD Biosciences (US, cat. #612563) and used 1:100. Rat anti-tyrosinated tubulin was from Abcam (cat. #ab6160) and used 1:200. Mouse anti-alpha tubulin was from Sigma-Aldrich (clone α-3A1, cat. #T5168) and used 1:400. The corresponding secondary antibodies for widefield were Alexa Fluor 488 and 568 (Thermo Fisher Scientific, anti-mouse-AF488 cat. # A32723, anti-rat-AF568 cat. #A11077); and anti-mouse ATTO-647N was used for STED (Sigma-Aldrich, cat. #50185).

Martínez G.F., Gazal N.G., Quassollo G., Szalai A.M., Cid-Pellitero E.D., Durcan T.M., Fon E.A., Bisbal M., Stefani F.D, & Unsain N. (2020). Quantitative expansion microscopy for the characterization of the spectrin periodic skeleton of axons using fluorescence microscopy. Scientific Reports, 10, 2917.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!