Anti phospho ampk thr172 2535

Manufactured by Cell Signaling Technology

Anti-phospho-AMPK Thr172 (2535) is a rabbit monoclonal antibody that recognizes AMPK (AMP-activated protein kinase) when phosphorylated at threonine 172. It is intended for use in various research applications, including western blotting, immunohistochemistry, and immunoprecipitation.

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Lab products found in correlation

Anti lc3b, by Merck Group (1 mentions) N acetyl l cysteine, by Fujifilm (1 mentions) Rapamycin, by Selleck Chemicals (1 mentions) Trehalose, by Merck Group (1 mentions) Anti actb, by Merck Group (1 mentions) Brefeldin a, by Merck Group (1 mentions) Anti rpn1, by Santa Cruz Biotechnology (1 mentions) Bafilomycin a1, by Fujifilm (1 mentions) Ac yvad cmk, by Cayman Chemical (1 mentions) Atg5 sirna, by Santa Cruz Biotechnology (1 mentions) Rack1, by Santa Cruz Biotechnology (1 mentions) Goat anti rabbit igg ab205718, by Abcam (1 mentions) Beclin 1, by Santa Cruz Biotechnology (1 mentions) Gapdh, by Santa Cruz Biotechnology (1 mentions) β actin, by Santa Cruz Biotechnology (1 mentions) Anti mouse hrp, by Jackson ImmunoResearch (1 mentions)

3 protocols using anti phospho ampk thr172 2535

Cellular Stress and Autophagy Modulation

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6-hydroxydopamine hydrochloride (6-OHDA; H4381), catalase (C8531), rotenone (R8875), MRT68921 (SML1644), and trehalose (T9531) were obtained from Sigma-Aldrich. Bafilomycin A₁ (023–11641), benzoquinone (171–00242), H₂O₂ (080–01186), N-acetyl-L-cysteine (NAC; 017–05131), and Z-VAD(OMe)-FMK (zVAD; 269–02071) were from Wako. Brefeldin A (203729) was from Merck. Ac-YVAD-CMK (YVAD; 10014) was from Cayman Chemical Company. Rapamycin (S1039) was obtained from Selleck Chemicals. The following antibodies were from commercial sources: anti-PARK7/DJ-1 (ab4150), anti-CTSB (abcam, ab58802); anti-RPN1 (Santa Cruz Biotechnology, sc-12164); anti-FN1 (610077), anti-CAV1 (BD Biosciences, 610060); anti-VDAC1 (Merck, AP1059); anti-LMNA (2032), anti-CASP3 (9662), anti-ATG16L1 (8089), anti-phospho-AMPK Thr172 (2535), anti-phospho-ULK1 Ser555 (5869), anti-phospho-RPS6KB1/p70S6K Thr389 (Cell Signaling Technology, 9234); and anti-LC3B (L7543), and anti-ACTB (Sigma-Aldrich, A5441). Monoclonal antibody against PARK7 (Clone 3843) was prepared as previously described [62]. All other chemicals, of analytical grade, were obtained from Sigma-Aldrich or Wako.

Urano Y., Mori C., Fuji A., Konno K., Yamamoto T., Yashirogi S., Ando M., Saito Y, & Noguchi N. (2018). 6-Hydroxydopamine induces secretion of PARK7/DJ-1 via autophagy-based unconventional secretory pathway. Autophagy, 14(11), 1943-1958.

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Cholesterol Efflux Regulation via AMPK-Dependent Autophagy

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RPMI 1640 medium FBS (C0252) was acquired from Beyotime Biotechnology (Shanghai, China). Phorbol‐12‐Myristate13‐acetate (PMA; P1585) and 3‐methyladenine (3MA, 5 mmol/L; M9281) were purchased from Sigma‐Aldrich. RACK1 siRNA (sc‐36354), ATG5 siRNA (sc‐41445), and primary antibodies such as RACK1 (sc‐17754), beclin‐1 (sc‐11427), LC3 (sc‐398822), ABCA1 (sc‐53482), GAPDH (sc‐47724), β‐actin (sc‐47778) and AMPK (sc‐17754) were acquired from Santa Cruz Biotechnology. Other primary antibodies like anti‐AMPK (2793) and anti–phospho‐AMPK Thr172 (2535) from acquired from Cell Signaling Technology, while p62 primary antibody was supplied by R&D Systems. Acetylated LDL (Ac‐LDL; L8940), FGF21 (ab63277) and secondary goat anti‐mouse IgG‐HRP (ab97023) and goat anti‐rabbit IgG (ab205718) antibodies came from Abcam, while pEGFP‐LC3 was supplied by Cell Biolabs.

Xiaolong L., Dongmin G., Liu M., Zuo W., Huijun H., Qiufen T., XueMei H., Wensheng L., Yuping P., Jun L, & Zhaolin Z. (2020). FGF21 induces autophagy‐mediated cholesterol efflux to inhibit atherogenesis via RACK1 up‐regulation. Journal of Cellular and Molecular Medicine, 24(9), 4992-5006.

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AMPK and Bile Acid Regulation in Hepatocytes

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The following materials were used in this study: 1) MIC-1, the AMPK inhibitor Compound C, the AMPK activator AICAR (5-aminoimidazole-4-carboxamide-1-β-D-ribofuranosyl 5′-monophosphate), anti-CYP7A1 (SAB4301212) antibody, and anti-α-tubulin (T9026) antibody (Sigma-Aldrich, St. Louis, MO); 2) anti-phospho-JNK (Thr183/Tyr185) (9255), anti-phospho-ERK (Thr202/Tyr204) (4370), and anti-phospho-AMPK (Thr172) (2535) antibodies (Cell Signaling Technology, Beverly, MA); 3) anti-SREBP2 (NV100-74543) antibody (Novus Biologicals, Littleton, CO); 4) anti-CYP27A1 antibody (GTX103718) (GeneTex, San Antonio, TX); 5) anti-HMGCR antibody (ab174830) (Abcam, Cambridge, MA); 6) anti-bile salt export pump (BSEP; ABCB11) (sc-74500), anti-multidrug resistance 2 (MDR2; ABCB4) (sc-58221), anti-ATP-binding cassette transporter (ABCG) 5 (sc-517207), and anti-ABCG8 (sc-30111) antibodies (Santa Cruz Biotechnology, Santa Cruz, CA); and 7) anti-mouse-HRP (horseradish peroxidase) (115-036-003) and anti-rabbit-HRP (111-035-003) antibodies (Jackson Laboratory, Bar Harbor, ME).

Kim M.H., Lee E.J., Kim S.J., Jung Y.J., Park W.J, & Park I. (2023). Macrophage inhibitory cytokine-1 aggravates diet-induced gallstone formation via increased ABCG5/ABCG8 expression. PLOS ONE, 18(6), e0287146.

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