Purified mouse anti actin ab 5

Manufactured by BD

Purified Mouse Anti-Actin Ab-5 is a laboratory reagent used to detect the presence of actin, a structural protein found in eukaryotic cells. It is a monoclonal antibody produced in mice and purified for use in various cell and molecular biology applications.

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Lab products found in correlation

M8064, by Merck Group (1 mentions) Ab124770, by Abcam (1 mentions) Cell culture reagents, by Thermo Fisher Scientific (1 mentions) Acetyl p53 lys382 antibody, by Cell Signaling Technology (1 mentions) Restore western blot stripping reagent, by Thermo Fisher Scientific (1 mentions) Phospho p53 ser15 antibody, by Cell Signaling Technology (1 mentions) Hrp conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions) Monoclonal anti α tubulin clone dm1a, by Merck Group (1 mentions) Prolong gold antifade reagent with dapi, by Thermo Fisher Scientific (1 mentions) Sp600125, by Merck Group (1 mentions) Protein g sepharose, by GE Healthcare (1 mentions)

3 protocols using purified mouse anti actin ab 5

Western Blot Analysis of Cytoskeletal Proteins

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Western blots were performed as previously described (40 (link)), using NuPage 3 to 8% or 4 to 12% gels and the following antibodies:
Primary antibodies: anti-MIIA antibody (rabbit) (1:1000; M8064, Sigma Aldrich), anti-MIIB antibody (N-17) (rabbit) (1:1000; 3404S, Cell Signaling), and anti-DIA1 antibody (E-4) (mouse) (1:100; SC-373807, Santa Cruz Biotechnology). β-Actin was used as a loading control (1:10,000; 612656; Purified Mouse Anti-Actin Ab-5, BD Biosciences).
Secondary antibodies: anti-mouse immunoglobulin G (IgG), horseradish peroxidase (HRP)–linked antibody (1:2000; 7076S, Cell Signaling), and anti-rabbit IgG, HRP-linked antibody (1:2000; 7074S, Cell Signaling).

Zhao R., Afthinos A., Zhu T., Mistriotis P., Li Y., Serra S.A., Zhang Y., Yankaskas C.L., He S., Valverde M.A., Sun S.X, & Konstantopoulos K. (2019). Cell sensing and decision-making in confinement: The role of TRPM7 in a tug of war between hydraulic pressure and cross-sectional area. Science Advances, 5(7), eaaw7243.

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Antibody Validation for SUN and Myosin Proteins

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Primary antibodies were anti-SUN1 antibody (produced in rabbit; Abcam; ab124770; 1:1,000), anti-SUN2 antibody (rabbit; Abcam; ab124916; 1:1,000), anti-MIIA antibody (rabbit; Sigma-Aldrich; M8064; 1:1,000), and anti-MIIB antibody (N-17; goat; Santa Cruz Biotechnology; SC-47205; 1:7,500). β-Actin was used as a loading control (Purified Mouse Anti-Actin Ab-5; BD Biosciences; 612656; 1:10,000).

Mistriotis P., Wisniewski E.O., Bera K., Keys J., Li Y., Tuntithavornwat S., Law R.A., Perez-Gonzalez N.A., Erdogmus E., Zhang Y., Zhao R., Sun S.X., Kalab P., Lammerding J, & Konstantopoulos K. (2019). Confinement hinders motility by inducing RhoA-mediated nuclear influx, volume expansion, and blebbing. The Journal of Cell Biology, 218(12), 4093-4111.

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Comprehensive Biochemical Reagents for Cell Culture

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Cell culture reagents were purchased from Life Technologies. SP600125 (1,9-Pyrazoloanthrone), MTT (3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2-tetrazolium-bromide), Monoclonal anti-β-Catenin antibody 6F9, Monoclonal Anti-α-Tubulin clone DM1A, Monoclonal Anti-Acetylated Tubulin Clone 6-11B-1 were purchased from Sigma-Aldrich. p53 antibody, Phospho-p53 (Ser46) antibody, p53 (1C12) Mouse mAb, p21 Waf1/Cip1 (DCS60) Mouse mAb, p21 Waf1/Cip1 (12D1) Rabbit mAb, Acetyl-p53 (Lys382) antibody, Phospho-p53 (Ser15), antibody, Phospho-Histone H2A.X 20E3 antibody, HDAC6 D2E5 antibody and Acetyl- β-Catenin (Lys49) antibody were purchased from Cell Signaling Technologies. ProLong Gold Antifade Reagent with DAPI, Wheat Germ Agglutinin Alexa-Fluor 594 Conjugate, Alexa-fluor conjugated and HRP conjugated secondary antibodies were purchased from Life Technologies. Purified Mouse Anti-Actin Ab-5 was purchased from BD Biosciences. Ischemin (MS120) and P-MYPT antibody were purchased from Merk Millipore. Phospho-Histone H3 pSer10 Antibody, TPPP/p25 antibody, TPPP antibody, Restore Western Blot Stripping reagent were purchased from Thermo Scientific. Protein G sepharose was purchased from GE Healthcare Life Sciences.

Grassi E.S., Vezzoli V., Negri I., Lábadi Á., Fugazzola L., Vitale G, & Persani L. (2015). SP600125 has a remarkable anticancer potential against undifferentiated thyroid cancer through selective action on ROCK and p53 pathways. Oncotarget, 6(34), 36383-36399.

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