Total RNA was obtained from cell samples (1×106) by TRNzol reagent and was transcribed to cDNA by MMLV reverse transcriptase kit. Then the expression levels of mRNA were measured on a BioRad real-time PCR instrument using SYBR green qPCR-mix kit and normalized to GAPDH.
Sybr green qpcr mix kit
Manufactured by Bio-Rad
The SYBR green qPCR-mix kit is a reagent used for quantitative real-time polymerase chain reaction (qPCR) analysis. It contains SYBR green, a fluorescent dye that binds to double-stranded DNA, and all the necessary components for qPCR amplification and detection.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using sybr green qpcr mix kit
1
Quantification of mRNA Expression
2
RNA Extraction and Real-Time qPCR
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Total RNA was obtained from cell samples (1×10 6 ) by TRNzol reagent and was transcribed to cDNA by MMLV reverse transcriptase kit. Then the expression levels of mRNA were measured on a BioRad real-time PCR instrument using SYBR green qPCR-mix kit and normalized to GAPDH.
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