4 6 diamidino 2 phenylindole dapi triton x 100

The DMEM medium and antibiotics (penicillin and streptomycin) were purchased from the HyClone Co. (Shanghai, China); PBS and CCK-8 kits were acquired from the Meilun Biotech (Dalian, China); 4′,6-diamidino-2-phenylindole (DAPI), Triton X-100, BCA protein assay reagent, PVDF membrane, and JC-1 kit were obtained from Beyotime (Haimen, China); Annexin V-FITC/PI kit was purchased from BD Biosciences (San Diego, CA, USA); FBS was purchased from the Tianhang Biotech (Hangzhou, China); cOmplete protease inhibitors, phosphatase inhibitors, and pronase were purchased from the Roche Co. (Shanghai, China); primary antibodies for Bcl-xL, Bim, Apaf-1, cleaved PARP, cleaved caspase-3, cleaved caspase-8, cleaved caspase-9, Akt, phosphorylation- (p-) Akt, PI3K, p-PI3K, JNK, p-JUK, ERK, p-ERK, p38, p-p38, CCAR1, Cyr61, β-actin, cytochrome c, and goat-anti-rabbit/rat horseradish-peroxidase- (HRP-) conjugated secondary antibodies were purchased from the Cell Signaling Technology Co. (Danvers, MA, USA); primary antibody for GAPDH was purchased from the Servicebio Co. (Wuhan, China); RED-NHS protein labeling kit was purchased from the NanoTemper Technologies (Munich, Germany); Cyr61 protein (Mammalian, C-Fc) was acquired from the Novoprotein Co. (Beijing, China); and sodium dodecyl sulfate (SDS) and loading buffer were acquired from Sangon Biotech (Shanghai, China).