Polyclonal anti ha antibody sc 805

Manufactured by Santa Cruz Biotechnology

The Polyclonal anti-HA antibody (SC-805) is a reagent used for the detection and identification of proteins that have been engineered to include the hemagglutinin (HA) epitope tag. The antibody is produced by immunizing animals with the HA peptide sequence and purifying the resulting polyclonal antibodies.

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Anti-HA (247 citations) Anti-HA antibody (91 citations) Sc-805 (41 citations) Anti-HA (sc-805, (16 citations) HA (sc-805, (6 citations) Polyclonal anti-HA (SC-805) (2 citations) Anti-HA antibody (sc-805) (2 citations) HA antibody (SC-805) (2 citations)

4 protocols using polyclonal anti ha antibody sc 805

Antibody Source and Dilution Protocol

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Anti-c-Myc antibody (SC-40, 1:1000), polyclonal anti-HA antibody (SC-805, 1:1000), anti-Cullin-1 antibody (SC-70895, 1:1000), and anti-Cyclin E antibody (SC-247, 1:1000) were purchased from Santa Cruz. Polyclonal anti-FLAG antibody (F2425, 1:1000), monoclonal anti-FLAG antibody (F-3165, 1:1000), anti-Vinculin antibody (V9131, 1:5000), peroxidase-conjugated anti-mouse secondary antibody (A4416, 1:3000), peroxidase-conjugated anti-rabbit secondary antibody (A4914, 1:3000) and CK1 inhibitor IC261 were purchased from Sigma. Anti-Myc-tag (2272, 1:1000), anti-GST (2625, 1:1000), anti-Vimentin (5741, 1:1000), anti-Snail (3879, 1:1000), anti-ZEB-1 (3396, 1:1000), anti-Twist1 (4119, 1:1000), anti-β-catenin (9582, 1:1000), anti-Arid1a (12354, 1:1000) and anti-BRM (11966, 1:1000) antibodies were purchased from Cell Signaling. Anti-FBW7 antibody (A301-720A, 1:1000) was purchased from Bethyl. Monoclonal anti-HA antibody (MMS-101P, 1:1000) was purchased from Covance. Anti-GFP antibody (632380, 1:1000) was purchased from Invitrogen.

Huang L.Y., Zhao J., Chen H., Wan L., Inuzuka H., Guo J., Fu X., Zhai Y., Lu Z., Wang X., Han Z.G., Sun Y, & Wei W. (2018). SCFFBW7-mediated degradation of Brg1 suppresses gastric cancer metastasis. Nature Communications, 9, 3569.

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Antibody Dilution Protocol for Western Blotting

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All antibodies were used at a 1:1000 dilution in TBST (tris-buffered saline–Tween 20) buffer with 5% nonfat milk for Western blotting. Anti–phospho-Ser⁴⁷³-Akt antibody (4060), anti-Akt1 antibody (2938), anti–total Akt antibody (4691), anti–phospho-S6 antibody (4858), anti-S6 antibody (2217), anti–phospho-eIF4B (Ser⁴⁰⁶) antibody (8151), anti-eIF4B antibody (3592), anti–His-tag antibody (12698), anti–phospho-S6K1 (Thr³⁸⁹) antibody (9205), and anti-S6K1 antibody (2708) were obtained from Cell Signaling Technology. Polyclonal anti-HA antibody (sc-805) was obtained from Santa Cruz Biotechnology. Anti-tubulin antibody (T-5168), anti-HA agarose beads (A-2095), peroxidase-conjugated anti-mouse secondary antibody (A-4416), and peroxidase-conjugated anti-rabbit secondary antibody (A-4914) were obtained from Sigma. Monoclonal anti-HA antibody (MMS-101P) was obtained from Covance.

Zhang J., Guo J., Qin X., Wang B., Zhang L., Wang Y., Gan W., Pandolfi P.P., Chen W, & Wei W. (2018). The p85 isoform of the kinase S6K1 functions as a secreted oncoprotein to facilitate cell migration and tumor growth. Science signaling, 11(523), eaao1052.

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Antibody Dilution and Specificity

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All antibodies were used at a 1:1000 dilution in 5% non-fat milk for western blot. Anti-EglN2 (NB100-310) and anti-EglN3 (NB100-139) antibodies were obtained from Novus biological. Anti-SPOP (16750-1-AP) antibody was purchased from Proteintech. Anti-EglN1 (4835), anti-Cullin 3 (2759), anti-GST (2625), anti-GFP (2956), anti-AR (5153) and anti-Myc-Tag (2278) antibodies were purchased from Cell Signaling. Polyclonal anti-HA (SC-805) antibody was purchased from Santa Cruz. Monoclonal anti-Flag (F-3165, clone M2), anti-Tubulin (T-5168), anti-Vinculin (V-4505) antibodies, anti-Flag agarose beads (A-2220), anti-HA agarose beads (A-2095), peroxidase-conjugated anti-mouse secondary antibody (A-4416) and peroxidase-conjugated anti-rabbit secondary antibody (A-4914) were purchased from Sigma. Monoclonal anti-HA (MMS-101P) antibody was purchased from Covance.

Zhang L., Peng S., Dai X., Gan W., Nie X., Wei W., Hu G, & Guo J. (2017). Tumor suppressor SPOP ubiquitinates and degrades EglN2 to compromise growth of prostate cancer cells. Cancer letters, 390, 11-20.

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Western Blot Antibody Validation

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Anti-IKZF1 (ab191394) antibody was purchased from Abcam. Anti-IKZF3 (NBP22449) antibody was purchased from Novus Biologicals. Anti-BRD3 (11859-1-AP) antibody was purchased from Proteintech. Anti-BRD4 (A301-985A-M) antibody was purchased from Bethyl Laboratories. Anti-ALK (3633) was purchased from Cell Signaling Technologies. Monoclonal anti-HA antibody (MMS-101P) was purchased from BioLegend. Polyclonal anti-HA (sc-805) antibody was purchased from Santa Cruz Biotechnologies. Anti-GFP antibody (632380) was purchased from Invitrogen. Polyclonal anti-Flag antibody (F-2425), monoclonal anti-Flag antibody (F-3165, clone M2), anti-tubulin antibody (T-5168), anti-vinculin antibody (V-4505), anti-Flag agarose beads (A-2220), anti-HA agarose beads (A-2095), peroxidase-conjugated anti-mouse secondary antibody (A-4416), and peroxidase-conjugated anti-rabbit secondary antibody (A-4914) were purchased from Sigma-Aldrich. All antibodies were used at a 1:1000 dilution in 5% bovine serum albumin in tris-buffered saline and tween 20 (TBST) buffer for Western blots.

Liu J., Chen H., Ma L., He Z., Wang D., Liu Y., Lin Q., Zhang T., Gray N., Kaniskan H.Ü., Jin J, & Wei W. (2020). Light-induced control of protein destruction by opto-PROTAC. Science Advances, 6(8), eaay5154.

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