Mwco 100 kda

Manufactured by Sartorius

The MWCO 100 kDa is a membrane-based filtration device used for the separation and purification of molecules based on their molecular weight. It is designed to retain molecules larger than 100 kilodaltons (kDa) while allowing smaller molecules to pass through.

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Lab products found in correlation

Coomassie plus bradford assay, by Thermo Fisher Scientific (2 mentions) Zetaview, by Particle Metrix (2 mentions)

2 protocols using mwco 100 kda

Isolation and Characterization of hESC-MSC Extracellular Vesicles

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The hESC‐MSC EVs were prepared as previously described (Lai et al., 2016). Briefly, immortalized hES‐ MSCs were grown in a chemically defined medium for 3 days and CM was harvested and pre‐cleared with a 0.22 µm syringe filter. The CM was concentrated 100× for exosomes by tangential flow filtration (Sartorius; MWCO 100 kDa) and stored in −20°C freezer until use. The EVs were assayed for protein concentration using Coomassie Plus (Bradford) Assay (Thermo Fisher Scientific) per manufacturer's instruction and characterized for particle size distribution and concentration by Zetaview (Particle Metrix) according to the manufacturer's protocol. Follow‐up experiments from subsequent batches of hESC‐MSC EVs (posterior to batch AC83) yielded identical results.

Dorronsoro A., Santiago F.E., Grassi D., Zhang T., Lai R.C., McGowan S.J., Angelini L., Lavasani M., Corbo L., Lu A., Brooks R.W., Garcia‐Contreras M., Stolz D.B., Amelio A., Boregowda S.V., Fallahi M., Reich A., Ricordi C., Phinney D.G., Huard J., Lim S.K., Niedernhofer L.J, & Robbins P.D. (2021). Mesenchymal stem cell‐derived extracellular vesicles reduce senescence and extend health span in mouse models of aging. Aging Cell, 20(4), e13337.

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Immortalized MSC Exosome Isolation

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MSC exosome was prepared as previously described [35, 36, 39 . Briefly, immortalized human embryonic stem cell-derived MSCs were grown in a chemically defined medium for 3 days and the conditioned medium was harvested and 0.22-mm filtered. The conditioned medium was concentrated 100 × for exosomes by tangential flow filtration (Sartorius; MWCO 100 kDa) and stored in -20°C freezer until use. The exosomes were assayed for protein concentration using Coomassie Plus (Bradford) Assay (Thermo Fisher Scientific), as per manufacturer's instruction, and characterized for particle size distribution and concentration by Zetaview (Particle Metrix) according to the manufacturer's protocol. The mean protein and particle contents of the exosome preparations were 1.9 mg/mL and 1.9 × 10 11 particles/mL.

Zhang B., Yeo R.W.Y., Lai R.C., Sim E.W.K., Chin K.C, & Lim S.K. (2018). Mesenchymal stromal cell exosome-enhanced regulatory T-cell production through an antigen-presenting cell-mediated pathway. Cytotherapy, 20(5).

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