Ethical guidelines were followed during all animal studies, as approved by the Ethics Committee of Wannan Medical College (No. 2021012). Ad-FLuc was provided by Prof. Tong-Chuan HE (University of Chicago). HCT116 and HCT116-siKCN cells were infected with Ad-Fluc to produce HCT116-FLuc and HCT116-siKCN-FLuc, respectively. 4–6-week-old BALB/c nude mice (male/female ratio 1:1) were subcutaneously injected with a total of 1.5 × 107 cells per injection were collected and injected subcutaneously into the right dorsal flank of the mice in 200 mL of PBS (4 mice per group) for the tumorigenesis assay. The animals were intraperitoneally injected with luciferase (ab145164, Abcam) after 2 and 14 days of injection, and bioluminescence imaging was performed using the Berthold LB983 imaging system. INDIGO software (Berthold Technologies) was used to analyze the data from bioluminescence images. The mice were euthanized after 20 days of injection and the tumors were collected and analyzed after making paraffin sections. Immunofluorescence staining using anti-PCNA (1:400, ab92552, Abcam) and anti-CCNE2 (1:400, ab40890, Abcam) was conducted to detect and quantify positive cells in three randomly selected high-power fields of each section.
Lb983 imaging system
Manufactured by Berthold Technologies
Sourced in Germany
The LB983 imaging system is a lab equipment product offered by Berthold Technologies. It is designed for imaging applications. The core function of the LB983 imaging system is to capture and process images.
Automatically generated - may contain errors
Lab products found in correlation
Indigo software, by Berthold Technologies (1 mentions)
Ab92552, by Abcam (1 mentions)
Ab40890, by Abcam (1 mentions)
Lsm 900 confocal laser scanning microscope, by Zeiss (1 mentions)
Cary eclipse fluorescence spectrophotometer, by Agilent Technologies (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
6550 qtof instrument, by Agilent Technologies (1 mentions)
Rpmi 1640 media, by Keygen Biotech (1 mentions)
3 protocols using lb983 imaging system
1
Tumorigenesis Assay of HCT116 Cells
2
Oxygen-Producing Nanoparticle Fluorescence and Photoacoustic Imaging
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To test the fluorescence (FL) imaging capability of multi-functional oxygen-producing nanoparticles, 200 μL of samples with CP-CLs@O2 and different concentrations of ICP-CLs@O2 were tested. The LB983 imaging system was used to capture the FL imaging (Berthold Technologies GmbH & Co. KG, Germany).
The agar gel model was supplemented with various concentrations of ICP-CLs@O2 to identify the photoacoustic (PA) imaging capability. The Vevo LAZR PA Imaging System was used to obtain the PA imaging (Visual Sonics Inc., Toronto, Canada).
The agar gel model was supplemented with various concentrations of ICP-CLs@O2 to identify the photoacoustic (PA) imaging capability. The Vevo LAZR PA Imaging System was used to obtain the PA imaging (Visual Sonics Inc., Toronto, Canada).
3
Organelle-Targeted Molecular Probes for Cell Death
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2-Methylbenzothiazole,
4-hydroxyisophthalaldehyde, rhodamine 123, and 1,4-dioxane were purchased
from Energy Chemical (China). LysoTracker Green was purchased from
Beyotime Biotechnology Co., Ltd. (Shanghai, China). Thiazolyl blue
tetrazolium bromide (MTT) was purchased from Biosharp. Erastin and
(1S, 3R)-RSL3 (RSL3) were obtained
from Target Molecule. Dulbecco′s modified Eagle medium (DMEM)
and fetal bovine serum (FBS) were purchased from Gibco Life Technologies.
Trypsin and DiO were obtained from Beijing Solarbio Science &
Technology Co., Ltd. Newborn calf serum was purchased from Zhejiang
Tianhang Biological Technology Co., Ltd. RPMI 1640 media were purchased
from KeyGEN BioTECH Co., Ltd.
NMR spectra were recorded on an
Agilent DD2 400 MHz spectrometer. High-resolution mass spectra (HR-MS)
were obtained using an Agilent 6550 Q-TOF instrument. UV–vis
absorption spectra were performed on a TU-1901 spectrophotometer (Beijing,
China). Fluorescence spectra were recorded on a Cary Eclipse fluorescence
spectrophotometer (Varian). The MTT assay was measured by a WD-2102B
microplate reader (Liuyi, Beijing, China). Fluorescence images were
carried out on an LSM 900 confocal laser scanning microscope (CarlZeiss,
Germany) with a 63× oil-immersion objective lens and processed
with ZEN software or ImageJ software. Mice imaging was performed on
a Nightowl LB 983 imaging system (Berthold, Germany).
4-hydroxyisophthalaldehyde, rhodamine 123, and 1,4-dioxane were purchased
from Energy Chemical (China). LysoTracker Green was purchased from
Beyotime Biotechnology Co., Ltd. (Shanghai, China). Thiazolyl blue
tetrazolium bromide (MTT) was purchased from Biosharp. Erastin and
(1S, 3R)-RSL3 (RSL3) were obtained
from Target Molecule. Dulbecco′s modified Eagle medium (DMEM)
and fetal bovine serum (FBS) were purchased from Gibco Life Technologies.
Trypsin and DiO were obtained from Beijing Solarbio Science &
Technology Co., Ltd. Newborn calf serum was purchased from Zhejiang
Tianhang Biological Technology Co., Ltd. RPMI 1640 media were purchased
from KeyGEN BioTECH Co., Ltd.
NMR spectra were recorded on an
Agilent DD2 400 MHz spectrometer. High-resolution mass spectra (HR-MS)
were obtained using an Agilent 6550 Q-TOF instrument. UV–vis
absorption spectra were performed on a TU-1901 spectrophotometer (Beijing,
China). Fluorescence spectra were recorded on a Cary Eclipse fluorescence
spectrophotometer (Varian). The MTT assay was measured by a WD-2102B
microplate reader (Liuyi, Beijing, China). Fluorescence images were
carried out on an LSM 900 confocal laser scanning microscope (CarlZeiss,
Germany) with a 63× oil-immersion objective lens and processed
with ZEN software or ImageJ software. Mice imaging was performed on
a Nightowl LB 983 imaging system (Berthold, Germany).
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