Sodium nitrite p a

Methanol (Ceamed, Lda., Funchal), Folin and Ciocalteu’s phenol reagent (Biochem Chemopharma, Cosne-Cours-sur-Loire, France), sodium hydroxide p.a. (Eka, Netherlands), sodium acetate (Honeywell, Charlotte, CA, USA), sodium nitrite p.a. (Merck, Darmstadt, Germany), indigo carmine (labkem, Spain), potassium permanganate (Acofarma, Madrid, Spain), sodium carbonate anhydrous p.a., potassium chloride (Panreac, Spain), absolute ethanol and sulfuric acid 95–97% (Chem-Lab, Zedelgem, Belgium), gallic acid (GA), catechin (CAT), aluminum chloride (99%), and 2,2-diphenyl-1-picrylhydrazyl (DPPH), purchased from Sigma-Aldrich (St. Louis, MO, USA), were used as chemicals for the extractions and bioactive characterization of the extracts.
Agar Powder (VWR, Lutterworth, UK); amphotericin B solution (Sigma, USA); blank, kanamycin (K) 30UG, and penicillin G (P) 2IU discs (LIOFILCHEM, Roseto degli Abruzzi, Italy); D(+)-glucose monohydrate and yeast extract (Scharlau, Barcelona, Spain); dimethyl sulfoxide (DMSO) and Mueller-Hinton Agar (Merck, Germany); Nutrient Agar (Biolab, Budapest, Hungary); and Nutrient Broth and Peptone (Cultimed, Spain) were used as chemicals for antimicrobial characterization of the extracts.

1 mol/L hydrochloric acid, 1 mol/L sodium hydroxide solutions and pH 7 and pH 10 buffer solutions were purchased from Carl Roth GmbH + Co. KG (Karlsruhe, Germany). Sodium nitrite p.a. was obtained from Merck KGaA (Darmstadt, Germany). The MWF concentrate “Curtis HiSpeed 441 Eco” used in this paper was produced by Curtis Systems GmbH (Hochheim, Germany).