Molecular sieves 3a

DNA from salmon testes was purchased from Tokyo Chemical Industry CO., LTD. Ultrapure water for inkjet spotting and sensing measurements was prepared by Millipore Milli-Q. Acetone, methanol, ethanol, acetonitrile, n-hexane, benzene, toluene, tetrahydrofuran (THF), and pyridine for sensing measurements were purchased from FUJIFILM Wako Pure Chemical Corporation. All solvents were dehydrated (H₂O < 0.001–0.005%). Molecular sieves 3A were purchased from FUJIFILM Wako Pure Chemical Corporation. Unless otherwise noted, the materials were used as purchased.

The natural DNA phosphoramidites and 8-aza-7-deaza-2´-deoxyguanosine phosphoramidite were purchased from Glen Research. 7-Deaza-2´-deoxyguanosine phosphoramidite was purchased from Chem Genes. The synthetic reagents [activators: 1H-tetrazole, dicyanoimidazole, 5-benzylthio-1H-tetrazole; capping reagents: acetic anhydride, phenoxyacetic anhydride, 1-methylimidiazole; oxidation reagents: iodine, (1S)-(+)-(10-camphorsulfonyl)-oxaziridine; deblocking reagents: dichloroacetic acid; solid support: Glen UnySupport] were all from Glen Research. Anhydrous acetonitrile, molecular sieves 3A, triethylamine, and 3% trichloroacetic acid in dichloromethane were from Fujifilm-Wako. Ammonium hydroxide was from Sigma-Aldrich. Q5 High-Fidelity DNA polymerase and Phusion High-Fidelity DNA polymerase were from New England Biolabs. TaKaRa Ex Taq was from TaKaRa Bio. Indexed adapter oligonucleotides were from Integrated DNA Technologies (IDT). PCR primers were from Eurofins.