Prism 7 software statistical package

Results are expressed as mean ± standard error of mean (SEM). Following assessment for normality and equality of variances, statistical inferences on data were performed using one-way analysis of variance (ANOVA) followed by pairwise comparisons of treatment means using Dunnett’s post-hoc test (vs. unstimulated controls or vs. stimuli used in the inhibition studies). Differences were considered statistically significant when p < 0.05. Luciferase activity represented as area under the curve (AUC) and soluble TNF secreted levels were evaluated using Pearson’s correlation analysis. Statistical analyses were performed using GraphPad Prism 7 Software Statistical Package, La Jolla CA; USA.

Results were represented as the mean ± standard error of the mean (SEM). Following assessment for normality and equality of variances, statistical inferences on data were performed using one-way, or two-way analysis of variance (ANOVA) followed by unpaired comparisons of treatment means using Dunnett's post-hoc test (LPS-treated or vs. LPS+IL-10) used in the luciferase inhibition assay and (untreated or vs. treated) used in the stability study. Differences were considered statistically significant when p < 0.05. Luciferase activity represented as area under the curve (AUC) Statistical analyses were performed using GraphPad Prism-7 Software Statistical Package, La Jolla CA; the USA.