If not stated otherwise, all plectasin samples were dialyzed into the desired condition using Slide-A-Lyzer TM 2000 MWCO dialysis cassettes (Thermo Fisher) with a buffer exchange after 2 and 4 hours and then continued overnight, ensuring a 200-fold dilution each step. The protein concentration after dialysis was measured using a NanoDrop™ 8000 Spectrophotometer (ThermoFisher). If not stated otherwise, all measurements were performed at a protein concentration of 2 mg/ml (455µM), obtained by dilution into the final condition. Filtration of the sample and buffer was performed using Luer-Lok TM syringes (BD) and 0.22µm Millex®-GV filter (Merck) or 0.02µm Whatman Anotop TM 10 filter (GE Healthcare Life Science). The pH reported was measured on the dialysis buffer if not otherwise stated.
Slide a lyzer tm 2000 mwco dialysis cassettes
Manufactured by Thermo Fisher Scientific
The Slide-A-Lyzer TM 2000 MWCO dialysis cassettes are a laboratory equipment product designed for dialysis applications. They have a molecular weight cutoff of 2000 Da and are intended for sample preparation and purification processes.
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2 protocols using slide a lyzer tm 2000 mwco dialysis cassettes
1
Plectasin Dialysis and Purification
2
Plectasin Dialysis and Characterization
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If not stated otherwise, all plectasin samples were dialyzed into the desired condition using Slide-A-LyzerTM 2000 MWCO dialysis cassettes (Thermo Fisher) with a buffer exchange after 2 and 4 h and then continued overnight, ensuring a dilution of at least 200 times in each step. The concentration of plectasin wildtype stock solution was c = 39 mg/mL (8.9 mM) and of PPI42 c = 37 mg/mL (8.4 mM). If not gelled, the protein concentration after dialysis was measured using a NanoDrop™ 8000 Spectrophotometer. The pH reported was the measured pH of the dialysis buffer, if not stated otherwise. Different buffers were chosen to ensure sufficient buffer capacity at different pH values. For the lower pH range (pH 3.5–5.5) sodium acetate buffer was chosen, if not stated otherwise. For the neutral pH range (pH 5–7.5) phosphate buffer was chosen, if not stated otherwise.
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