M-CSF, IL-3, and IFN-β were purchased from PeproTech (Rocky Hill, NJ). Anti-IFNAR2 neutralizing antibody was purchased from PBL Assay Science (Piscataway, NJ). Ultra-LEAF purified mouse IgG2a, κ isotype control antibody was purchased from BioLegend (San Diego, CA). USP18 [D4E7], p-STAT1-Tyr701 [58D6], p-STAT2-Tyr690 [D3P2P], and HSP90 [C45G5] monoclonal antibodies, β-actin and ISG15 polyclonal antibodies, and anti-mouse IgG, HRP-linked and anti-rabbit IgG, HRP-linked secondary antibodies were purchased from Cell Signaling Technology (Danvers, MA). Anti-HIV-1 p24 Antibody [39/5.4A] was purchased from Abcam (Cambridge, MA). CD68-PE [Y1/82A], CD11b-FITC [ICRF44], CD14-APC [61D3], and isotype control flow antibodies were purchased from (eBioscience (San Diego, CA).
Anti hiv 1 p24 antibody 39 5.4a
Manufactured by Abcam
The Anti-HIV-1 p24 Antibody [39/5.4A] is a mouse monoclonal antibody that recognizes the p24 core protein of HIV-1. It can be used for the detection of HIV-1 in a variety of immunoassays.
Automatically generated - may contain errors
Lab products found in correlation
Ifn β, by Thermo Fisher Scientific (2 mentions)
Anti ifnar2 neutralizing antibody, by PBL Assay Science (2 mentions)
β actin and isg15 polyclonal antibodies, by Cell Signaling Technology (2 mentions)
Interleukin 3 (il 3), by Thermo Fisher Scientific (2 mentions)
Isotype control flow antibodies, by Thermo Fisher Scientific (2 mentions)
M csf, by Thermo Fisher Scientific (2 mentions)
Raltegravir, by Santa Cruz Biotechnology (1 mentions)
Omega microplate reader, by BMG Labtech (1 mentions)
Luciferase assay system, by Promega (1 mentions)
3 protocols using anti hiv 1 p24 antibody 39 5.4a
1
Macrophage Differentiation and Signaling Assay
2
Interferon Signaling Pathway Activation
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M‐CSF, IL‐3, and IFN‐β were purchased from PeproTech (Rocky Hill, NJ). Anti‐IFNAR2 neutralizing antibody was purchased from PBL Assay Science (Piscataway, NJ). Ultra‐LEAF purified mouse IgG2a, κ isotype control antibody was purchased from BioLegend (San Diego, CA). USP18 [D4E7], p‐STAT1‐Tyr701 [58D6], p‐STAT2‐Tyr690 [D3P2P], and HSP90 [C45G5] monoclonal antibodies, β‐actin and ISG15 polyclonal antibodies, and anti‐mouse IgG, HRP‐linked and anti‐rabbit IgG, HRP‐linked secondary antibodies were purchased from Cell Signaling Technology (Danvers, MA). Anti‐HIV‐1 p24 Antibody [39/5.4A] was purchased from Abcam (Cambridge, MA). CD68‐PE [Y1/82A], CD11b‐FITC [ICRF44], CD14‐APC [61D3], and isotype control flow antibodies were purchased from (eBioscience, San Diego, CA).
3
Vpr Expression and Luciferase Assay
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The wild-type Vpr sequence was excised from pNL4.3 (ARP-3418, NIH AIDS Reagent Program) by digestion with PflMI and SalI restriction enzymes and ligated into pNL4-3.Luc.E−R− (ARP-114; NIH AIDS Reagent Program), digested in the same manner. Virus was prepared by transfection of 293T cells with these DNAs with a VSV-G expression construct. Before infection, the viral supernatants were checked for the presence and absence of Vpr by Western blot. Anti-Vpr polyclonal antibody (Proteintech, #51143-1-AP) and anti-HIV1 p24 antibody [39/5.4A] (Abcam, #ab9071) were used for staining. HeLa cells were treated with 10 µM Raltegravir (Santa Cruz Biotechnology, sc-364600) or the equivalent amount of dimethyl sulfoxide and infected with reporter viruses encoding Vpr or without Vpr. Luciferase activity was assessed 24 h after infection with the Luciferase Assay System (Promega, #E1501) according to the manufacturer’s instructions and measured on an Omega microplate reader (BMG Labtech).
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