Proteins were extracted by a nuclear protein and cytoplasmic protein extraction kit (Servicebio, Ghent, Belgium). Bicinchoninic acid (BCA) protein concentration assay kit (Servicebio) was used to measure protein concentration. Western blotting analyses were performed as usual procedures. Rabbit anti-human FASN polyclonal antibody (FASN) (abmart, Shanghai, China; 1:1,000), rabbit anti-human Beclin1 polyclonal antibody (Beclin1) (abmart, 1:1,000), and rabbit anti-human LAMP polyclonal antibody (Beclin1) (abmart, 1:1,000) were used as the primary antibodies for CCs. Rabbit anti-human ASAH polyclonal antibody (29 (link)) (abcam, Cambridge, UK; 1:1,000), rabbit anti-human Beclin1 polyclonal antibody (Beclin1) (abmart, 1:1,000), and rabbit anti-human LAMP polyclonal antibody (Beclin1) (abmart, 1:1,000) were used as the primary antibodies for MGCs. Goat anti-mouse IgG conjugated with horseradish peroxidase (HRP) was used as a secondary antibody (abclonal, 1:3,000). The density of the target bands was analyzed by the Alpha software processing system (30 (link)).
Turathum B., Gao E.M., Grataitong K., Liu Y.B., Wang L., Dai X, & Chian R.C. (2022). Dysregulated sphingolipid metabolism and autophagy in granulosa cells of women with endometriosis. Frontiers in Endocrinology, 13, 906570.
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