FT-IR spectroscopy was used to elucidate the chemical bonds and functional groups present in the BS structure (Pornsunthorntawee et al. 2008) (link).BS sample was analysed in Alpha FTIR spectrophotometer(Bruker, Germany), equipped with Opus graph plotter (Pornsunthorntawee et al. 2008 (link)). The lyophilized dry biosurfactant sample was mixed with potassium bromide (KBr) and compressed into a tablet form (Yalcin E 2010), scanned under 700 to 4000cm - 1 with the resolution of 4cm - 1 . NMR TLC puri ed BS sample was dissolved in deuterated water (D 2 O) in the concentration of 50µg/ml, and 1 H-NMR spectroscopy was done to determine the structural characteristics of the BSwith the instrument 'Bruker AFCEND-Germany spectrophotometer'. The scanning was done at the frequency of 400mHz (Makkar and Cameotra 1999) . The spectrograph was analysed with 'Mesternova' software.
Afcend germany spectrophotometer
Manufactured by Bruker
Sourced in Germany
The AFCEND-Germany spectrophotometer is a laboratory instrument designed to measure the absorption or transmission of light in a sample. It is capable of analyzing the spectral properties of various materials and solutions. The core function of this device is to provide accurate and reliable data on the optical characteristics of the samples under investigation.
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2 protocols using afcend germany spectrophotometer
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Characterization of Biosurfactant Structure
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Characterization of Biosurfactant Structure
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FT-IR spectroscopy was used to elucidate the chemical bonds and functional groups present in the BS structure (Pornsunthorntawee et al. 2008) (link).BS sample was analysed in Alpha FTIR spectrophotometer(Bruker, Germany), equipped with Opus graph plotter (Pornsunthorntawee et al. 2008 (link)). The lyophilized dry biosurfactant sample was mixed with potassium bromide (KBr) and compressed into a tablet form (Yalcin E 2010), scanned under 700 to 4000cm - 1 with the resolution of 4cm - 1 . NMR TLC puri ed BS sample was dissolved in deuterated water (D 2 O) in the concentration of 50µg/ml, and 1 H-NMR spectroscopy was done to determine the structural characteristics of the BSwith the instrument 'Bruker AFCEND-Germany spectrophotometer'. The scanning was done at the frequency of 400mHz (Makkar and Cameotra 1999) . The spectrograph was analysed with 'Mesternova' software.
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