Images of whole-mount nodulated roots were captured using a Leica stereo fluorescence microscope M205FA equipped with a Leica DFC310FX digital camera (Leica Microsystems). Detached nodules were embedded in Shandon cryomatrix (Thermo scientific) and were sliced into 30-µm thick sections with a rotary cryomicrotome CM1850 (Leica Microsystems). For confocal microscopy, sample preparation was done according to Haynes and associates (2004) . Sections were stained with Calcofluor (Life Technologies), propidium iodide (Life Technologies), and Syto9 (Life Technologies). Images were acquired with a Leica TCS SP5 II AOBS confocal laser scanning microscope (Leica Microsystems). For confocal and scanning electron microscopy, sample preparation was done according to (Kundu and DasGupta 2018) . All digital micrographs were processed using Adobe Photoshop CS5.
Tcs sp5 2 aobs confocal laser scanning microscope
Manufactured by Leica
The Leica TCS SP5 II AOBS is a confocal laser scanning microscope. It utilizes an Acousto-Optical Beam Splitter (AOBS) to provide adjustable laser excitation and emission detection.
Automatically generated - may contain errors
Lab products found in correlation
Propidium iodide, by Thermo Fisher Scientific (2 mentions)
Shandon cryomatrix, by Thermo Fisher Scientific (2 mentions)
Dfc310 fx digital camera, by Leica (2 mentions)
Calcofluor, by Thermo Fisher Scientific (2 mentions)
Syto9, by Thermo Fisher Scientific (2 mentions)
Fluozin 3 am, by Thermo Fisher Scientific (2 mentions)
Cm1850, by Leica (1 mentions)
M205 fa, by Leica (1 mentions)
4 protocols using tcs sp5 2 aobs confocal laser scanning microscope
1
Nodule Imaging and Microscopy Protocol
2
Whole-Mount Imaging of Nodulated Roots
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Images of whole-mount nodulated roots were captured using a Leica M205FA stereofluorescence microscope equipped with a Leica DFC310FX digital camera (Leica Microsystems). Detached nodules were embedded in Shandon cryomatrix (Thermo Scientific) and sliced into 30-µm-thick sections with a CM1850 rotary cryomicrotome (Leica Microsystems). For confocal microscopy, sample preparation was done according to Haynes and associates (2004) and Sinharoy and DasGupta (2009) . Sections were stained with Calcofluor (Life Technologies), propidium iodide (Life Technologies) and Syto9 (Life Technologies). Images were acquired with a Leica TCS SP5 II AOBS confocal laser-scanning microscope (Leica Microsystems). All digital micrographs were processed using Adobe Photoshop CS5.
3
Zn2+ Imaging in C2C12 Myoblasts
4
Zn2+ Imaging in C2C12 Myoblasts
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Proliferating and differentiating C2C12 cells were loaded with 2 μM Fluo-Zin3 AM, cell permeant (Invitrogen) in the corresponding culture media for 40 min at 37 °C. Fluo-Zin3 has a high affinity to bind Zn2+. Then, the cells were washed with fresh media and allowed to rest for another 20 min at 37 °C. For live imaging of Zn the media was exchanged for PBS right before microscopic analyses, to avoid fluorescent interference of the culture media. In addition, cells were fixed with 10% formalin-PBS and counterstained with DAPI. Fluorescence images were obtained with a Leica TCS SP5 II AOBS confocal laser-scanning microscope (Leica).
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