β actin antibody

Dulbecco's modified Eagle's medium (DMEM) and penicillin/streptomycin were purchased from Hyclone (Logan, USA). Foetal bovine serum (FBS) was purchased from Gibico (South America). HIF-1α antibody was purchased from Abcam (Cambridge, United Kingdom). VEGF antibody was purchased from RD (Minneapolis, USA). Antibodies against AKT, p-AKT, mTOR, p-mTOR, ERK, p-ERK, MMP2, and MMP9 were purchased from CST (Boston, USA). Horseradish peroxidase- (HRP-) conjugated secondary antibodies, fluorescent-conjugated secondary antibodies, New Super ECL Assay, and β-actin antibody were purchased from KeyGEN BioTECH (Nanjing, China). Transwell chambers and matrigel were purchased from Corning Life Sciences (8 μm pores, Tewksbury, MA, USA) and BD Biosciences (10.5mg/ml, San Jose, CA, USA), respectively. The QRT-PCR Kits were purchased from Takara (Shiga, Japan). The Cell Counting kit-8 (CCK-8) was purchased from Dojindo Molecular Technologies, Inc. (Kumamoto, Japan). The enzyme-linked immune sorbent assay (ELISA) kits for human VEGF were purchased from Multi Sciences (Shanghai, China). BCA Protein Assay Kits were purchased from Beyotime Biotechnology.