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Rat insulin elisa

Manufactured by DRG International
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Sourced in Germany

The Rat Insulin ELISA is a laboratory equipment used to measure the concentration of insulin in rat biological samples. It is a sensitive and specific immunoassay that employs the sandwich ELISA technique.

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Lab products found in correlation

Vacutainer k3e, by BD (1 mentions)

2 protocols using rat insulin elisa

1

Serum Insulin Quantification Protocol

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Venous blood was transferred into serum-vials, left on ice for 30 min and centrifuged for 10 min at 2000 g. The supernatant (serum) was stored at −80°C. Quantitative measurement of serum insulin levels was performed using a rat insulin ELISA (DRG Instruments GmbH, Marburg, Germany) and a rat insulin RIA (Cat. # SRI-13K, Millipore, St. Charles, Missouri, USA) according to the manufacturer's instructions.
Oelze M., Kröller-Schön S., Welschof P., Jansen T., Hausding M., Mikhed Y., Stamm P., Mader M., Zinßius E., Agdauletova S., Gottschlich A., Steven S., Schulz E., Bottari S.P., Mayoux E., Münzel T, & Daiber A. (2014). The Sodium-Glucose Co-Transporter 2 Inhibitor Empagliflozin Improves Diabetes-Induced Vascular Dysfunction in the Streptozotocin Diabetes Rat Model by Interfering with Oxidative Stress and Glucotoxicity. PLoS ONE, 9(11), e112394.
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2

Quantifying Plasma Hormones in Rats

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Venous blood was transferred into plasma vials (BD Vacutainer K3E with 250 KIU aprotinin), left on ice for 30 min and centrifuged for 10 min at 2000 g. The supernatant (plasma) was stored at –80 °C. Quantitative measurement of insulin and glucagon levels was performed using a rat insulin (#SRI-13K) or glucagon (#GLK-32) RIA (Merck (Millipore), St. Charles, Missouri, USA) according to the manufacturer's instructions. Insulin levels were also measured in serum, which was generated without aprotinin and EDTA, using a rat insulin ELISA (DRG Instruments GmbH, Marburg, Germany). Leptin and adiponectin levels were determined using ELISA (Leptin Quantikine Elisa Mouse / Rat Immunoassay, Cat.-Nr. MOB00, R&D Systems, Wiesbaden, Germany; Adiponectin (ADP) Elisa Mouse / Rat / Human, Cat.-Nr. MBS066180, MyBioSource, USA).
Steven S., Oelze M., Hanf A., Kröller-Schön S., Kashani F., Roohani S., Welschof P., Kopp M., Gödtel-Armbrust U., Xia N., Li H., Schulz E., Lackner K.J., Wojnowski L., Bottari S.P., Wenzel P., Mayoux E., Münzel T, & Daiber A. (2017). The SGLT2 inhibitor empagliflozin improves the primary diabetic complications in ZDF rats. Redox Biology, 13, 370-385.
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