Liver cancer cell lines of human, including HepG2, Huh-7, LM3, MHCC-97H, MHCC-97L, SK-Hep1, SMMC-7721, SNU-423 and SNU-475 were obtained from Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Liver cancer cells were cultured in a speci c culture medium according to ATCC instructions, supplemented with 10% (v/v) fetal bovine serum (FBS, Gibco, Grand Island, NY, USA) and 1% (v/v) penicillin (100 U/ml) and astreptomycin (0.1 mg/ml). All of above cells were incubated in a humidi ed incubator under 5% CO 2 at 37 °C. Vertepor n (VP, S1786) and Super-TDU (S8554) were purchased from Selleck (Shanghai, China). Huh-7 cells were treated with 50 nM Vertepor n or 50 nM Super-TDU (1-31), respectively.
Snu 423
Manufactured by National Collection of Authenticated Cell Cultures
Sourced in China
The SNU-423 is a laboratory equipment used for the cultivation and maintenance of cell cultures. It provides a controlled environment for the growth and propagation of various cell lines. The device ensures the necessary temperature, humidity, and gas composition required for the optimal growth and preservation of cultured cells.
Automatically generated - may contain errors
Lab products found in correlation
Sk hep1, by National Collection of Authenticated Cell Cultures (5 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (5 mentions)
Smmc 7721, by National Collection of Authenticated Cell Cultures (4 mentions)
Mhcc 97h, by National Collection of Authenticated Cell Cultures (4 mentions)
Mhcc 97l, by National Collection of Authenticated Cell Cultures (4 mentions)
Snu 475, by National Collection of Authenticated Cell Cultures (3 mentions)
Hepg2, by National Collection of Authenticated Cell Cultures (3 mentions)
Super tdu, by Selleck Chemicals (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Penicillin, by Thermo Fisher Scientific (2 mentions)
Streptomycin, by Thermo Fisher Scientific (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Super tdu s8554, by Selleck Chemicals (1 mentions)
Hcclm3, by National Collection of Authenticated Cell Cultures (1 mentions)
Snu 449, by National Collection of Authenticated Cell Cultures (1 mentions)
Vertepor n, by Selleck Chemicals (1 mentions)
Fetal bovine serum (fbs), by ScienCell (1 mentions)
Thle 3, by American Type Culture Collection (1 mentions)
Thle 2, by American Type Culture Collection (1 mentions)
6 protocols using snu 423
1
Liver Cancer Cell Line Cultivation
2
Cultivation of Human Liver Cancer Cell Lines
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Human HCC cell lines, including HCCLM3, HepG2, Huh-7, MHCC-97H, MHCC-97 L, SK-Hep1, SMMC-7721, SNU-423 and SNU-449 were purchased from Cell Bank of the Chinese Academy of Sciences. Dulbecco’s modified Eagle’s medium (DMEM) contained 10% (v/v) fetal calf serum (FCS) and 1% antibiotics was used. Cells were incubated at 37 °C in a humidified incubator under 5% CO2 condition.
3
Liver Cancer Cell Line Treatments
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Liver cancer cell lines of human, including HepG2, Huh-7, LM3, MHCC-97H, MHCC-97L, SK-Hep1, SMMC-7721, SNU-423 and SNU-475 were obtained from Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Liver cancer cells were cultured in a speci cculture medium according to ATCC instructions, supplementedwith 10% (v/v)fetal bovine serum (FBS,Gibco, Grand Island, NY, USA) and 1% (v/v)penicillin (100 U/ml) and astreptomycin (0.1 mg/ml).All of above cellswere incubated in a humidi ed incubatorunder 5% CO2 at 37°C.Vertepor n (VP, S1786) and Super-TDU (S8554) were purchased from Selleck (Shanghai, China).Huh-7 cells were treated with 50nM Vertepor n or 50nM Super-TDU , respectively.
4
Liver Cancer Cell Line Cultivation
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Liver cancer cell lines of human, including HepG2, Huh-7, LM3, MHCC-97H, MHCC-97 L, SK-Hep1, SMMC-7721, SNU-423 and SNU-475 were obtained from Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Liver cancer cells were cultured in a specific culture medium according to ATCC instructions, supplemented with 10% (v/v) fetal bovine serum (FBS, Gibco, Grand Island, NY, USA) and 1% (v/v) penicillin (100 U/ml) and astreptomycin (0.1 mg/ml). All of above cells were incubated in a humidified incubator under 5% CO2 at 37 °C. Verteporfin (VP, S1786) and Super-TDU (S8554) were purchased from Selleck (Shanghai, China). Huh-7 cells were treated with 50 nM Verteporfin or 50 nM Super-TDU (1–31), respectively.
5
Cell Line Cultivation for Experiments
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LO2, HepG2, Huh-7 and SNU-423 cell lines were obtained from the Cell Bank of Type Culture Collection of Chinese Academy of Sciences (Shanghai, China). All cells were cultured in Dulbecco's modified Eagle's medium (DMEM; Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS; ScienCell Research Laboratories, Inc., San Diego, CA, USA) and 1% Penicillin-Streptomycin (Thermo Fisher Scientific, Inc.) and maintained at 37°C with 50 ml/l CO2.
6
Hepatocellular Carcinoma Cell Lines and Clinical Samples
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HCC cell lines (Hep3B, HuH7, SNU398, SNU423, SNU429, Hep3G2, SK-HEP-1, and PLC/PRF/5) were all purchased from the Cell Bank of Type Culture Collection of Chinese Academy of Sciences (Shanghai, China). In addition, two healthy liver cell lines, THLE2 and THLE3, were purchased from the American Type Culture Collection, Manassas, VA, USA. All cells were maintained in a 6-well plate in RPMI-1640 medium (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% FBS (Thermo Fisher Scientific), 100 µg/mL streptomycin (Thermo Fisher Scientific), and 100 U/mL penicillin (Thermo Fisher Scientific) in a humidified 37°C incubator with 5% CO2.
Clinical samples, including HCC tissues and adjacent noncancerous hepatic tissues, were collected from 43 patients with HCC during surgeries from June 2010 to January 2018. Participating patients did not receive any chemotherapy or radiation treatment prior to surgery. Upon surgical removal, all tissues were preserved in liquid nitrogen prior to further processing.
Clinical samples, including HCC tissues and adjacent noncancerous hepatic tissues, were collected from 43 patients with HCC during surgeries from June 2010 to January 2018. Participating patients did not receive any chemotherapy or radiation treatment prior to surgery. Upon surgical removal, all tissues were preserved in liquid nitrogen prior to further processing.
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