Blood samples were collected before assessment of NVC responses, centrifuged at 3500 revolutions per minute for 15 min and processed for serum collection. IGF-1 levels were determined by enzyme-amplified chemiluminescence immunoassay (CLIA) using an IGF-1 assay kit (Siemens Healthcare Diagnostics, Los Angeles, USA, catalogue nr: L2KGF2) on a Siemens IMMULITE 2000 platform (Siemens Healthcare GmbH, Erlangen, Germany).
Immulite 2000 platform
Manufactured by Siemens
Sourced in United States
The IMMULITE 2000 platform is an automated immunoassay system designed for clinical laboratory testing. It is capable of performing a variety of immunoassay tests for the detection and measurement of specific analytes in patient samples.
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Lab products found in correlation
2 protocols using immulite 2000 platform
1
Serum IGF-1 Quantification Protocol
2
Fasting Biomarker Analysis Protocol
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A whole-blood fasting sample of 40 mL was collected from each participant. Blood samples were placed on ice and processed within 4 h into plasma, serum, whole blood, and buffy coat components, following which they were stored at −80°C at each study site until analysis. Complete blood counts were analyzed at the respective clinic sites. Biochemical analyses of all lipid and blood chemistry profiles were performed at PBRC. Serum chemistry panels were assayed through the use of a DXC600 instrument (Beckman Coulter, Inc.). Insulin was measured by immunoassay on an Immulite 2000 platform (Siemens Corporation). Measurements of fasting glucose, glycated hemoglobin (HbA1c), insulin, HOMA-IR, alanine transaminase (ALT), total cholesterol, HDL and LDL cholesterol, and serum triglycerides were used in the current analysis.
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