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2 protocols using hpmc lv grade e3

1

Formulation and Characterization of FLZ Delivery System

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FLZ was obtained as a gift from Aljazerah Pharmaceutical Company (Riyadh, Saudi Arabia). Hydroxypropyl methylcellulose low viscosity; viscosity of ~ 3 cP for 2% aqueous solution, MWT ~ 20,000 Da; (HPMC LV grade E3) was gained from Dow Chemical Co. (Midland, MI, USA). Kollicoat IR (KL) was obtained from BASF (Ludwigshafen, Germany). Pluronic F-127 (PL F127) was purchased from C.H. (Erbesloh, Krefeld, Germany). Xanthan gum (XG) was purchased from Sigma Chemical Co. (MO, USA). Polyvinyl pyrrolidone K30 (PVP; molecular weight 30,000 Da) was purchased from ISP Technologies, Inc. (NJ, USA). SLS was bought from Fisher Scientific International, Inc. (NH, USA). Carbopol 934 was obtained from Goodrich Chemical Co. (Poole, England). Sodium alginate was purchased from Sigma Chemical Co. (MO, USA). Standard dialysis cellophane membrane, molecular weight cut off ≈ 14,000 (Sigma chemical Co., MO, USA).The acetonitrile (HPLC–gradient) was purchased from Panreac (Madrid, Spain). Formic acid was purchased from Fisher Scientific (MA, USA).
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2

Glipizide Delivery System Formulation

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Glipizide was purchased from A.M.S.A. Anonima Materie Sintetiche and Afini (S.P.A., Como, Italy). Lipoid S100 (phosphatidylcholine (PC), soybean lecithin, >94% PC) was obtained from Lipoid GmbH (Ludwigshafen, Germany). Cholesterol and low-molecular-weight chitosan (viscosity 20,000 cps), degree of deacetylation (DD of 92%) were purchased from Sigma-Aldrich Chemical Co. Ltd. (St. Louis, MO, USA). Sodium deoxycholate, methanol, and acetonitrile (HPLC grade) were obtained from Fisher Scientific Co., (Hampton, NH, USA). Tween 80 was obtained from BDH Organics (England, UK). Hydroxypropyl methylcellulose (HPMC) low viscosity (viscosity of ~3 cP for 2% aqueous solution), MWT ~20,000 Da; (HPMC LV grade E3) was purchased from Dow Chemical Co. (Midland, MI, USA). Propylene glycol (PG) was obtained from WINLAB Laboratory chemical reagents (Leicestershire, UK). Streptozotocin and sodium carboxymethyl cellulose (average molecular weight of ~90,000) were purchased from Sigma-Aldrich (St. Louis, MO, USA). All the other chemicals were of analytical grade.
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