Human NSCLC A549 cells and SCLC Calu-6 cells were obtained from the American Type Culture Collection (Manassas, VA, USA). Primary normal HPF cells were purchased from PromoCell GmbH (C-12360, Heidelberg, Germany) and used between passages five and seven. These lung cells were kept in a standard humidified incubator at 37 °C with 5% CO2 and cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (Sigma-Aldrich Co., St. Louis, MO, USA) and 1% penicillin-streptomycin (Gibco BRL, Grand Island, NY, USA). Cells were grown in 100 mm plastic cell culture dishes (BD Falcon, Franklin Lakes, NJ, USA) and harvested with trypsin-EDTA (Gibco BRL).
Sclc calu 6 cells
Manufactured by American Type Culture Collection
Sourced in United States
The SCLC Calu-6 cells are a human lung cancer cell line derived from a small cell lung carcinoma. These cells are commonly used in research to study small cell lung cancer and related cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
100 mm plastic cell culture dishes, by BD (2 mentions)
Trypsin edta, by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Rpmi 1640 medium, by Merck Group (2 mentions)
Fetal bovine serum (fbs), by Merck Group (2 mentions)
A549 human nsclc cells, by American Type Culture Collection (1 mentions)
C 12360, by PromoCell (1 mentions)
Normal lung wi 38 va 13 subclone 2ra cells, by American Type Culture Collection (1 mentions)
2 protocols using sclc calu 6 cells
1
Culturing Human Lung Cell Lines
2
Culturing Lung Cancer and Normal Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Human NSCLC A549 adenocarcinoma cells, SCLC Calu-6 cells, and normal lung WI-38 VA-13 subclone 2RA cells were obtained from the American Type Culture Collection (Manassas, VA, USA). Primary normal HPF cells were purchased from PromoCell GmbH (C-12360, Heidelberg, Germany) and used between passages five and ten. Lung cells were kept in a standard humidified incubator at 37 °C with 5% CO2 and cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS; Sigma-Aldrich Co., St. Louis, MO, USA) and 1% penicillin–streptomycin (GIBCO BRL, Grand Island, NY, USA). Cells were grown in 100 mm plastic cell culture dishes (BD Falcon, Franklin Lakes, NJ, USA) and harvested with trypsin–EDTA (Gibco BRL).
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