3h pregnenolone

[³H]Pregnenolone, [³H]progesterone, [³H]androstenedione, [³H]testosterone, and [³H]dihydrotestosterone were purchased from DuPont-New England Nuclear (Boston, MA). Unlabeled pregnenolone, progesterone, 17α-hydroxyprogesterone, androstenedione, and testosterone were obtained from Steraloids (Newport, RI). Dibutyl phthalate and monobutyl phthalate were purchased from Sigma (St. Louis, MO). Male Sprague Dawley rats (30-day-old) were purchased from Shanghai Animal Center (Shanghai, China). All animal procedures were approved by the Institutional Animal Care and Use Committee of Wenzhou Medical University and were performed in accordance with the Guide for the Care and Use of Laboratory Animals.

[³H]Pregnenolone, [³H]progesterone, [³H]androstenedione, [³H]testosterone, [³H]dihydrotestosterone were purchased from DuPont-New England Nuclear (Boston, MA). Unlabeled pregnenolone, progesterone, 17α-hydroxyprogesterone, androstenedione and testosterone were obtained from Steraloids (Newport, RI). Etomidate was purchased from Sigma (St. Louis, MO). Male Sprague-Dawley rats (30-day-old) were purchased from Shanghai Animal Center (Shanghai, China). All animal procedures were approved by the Institutional Animal Care and Use Committee of Wenzhou Medical University and were performed in accordance with the Guide for the Care and Use of Laboratory Animals.

Rat LH standard NIDDK-r-LH-I9 was obtained through the National Hormone and Pituitary Program (United States). ³H-CORT, ³H-hydroxycholesterol, ³H-pregnenolone, ³H-progesterone, ³H-androstenedione, and ³H-T were purchased from DuPont-New England Nuclear (Boston, MA, United States). ³H-DHC was prepared as previously described (Lakshmi and Monder, 1985 (link)). The CORT antiserum B3-163 was obtained from Endocrine Sciences (Calabasas, CA, United States). NAD⁺ and NADPH were purchased from Sigma (St. Louis, MO, United States). RU486 was from Roussel (UCLAF, France). DHEA, 2-hydroxypropyl-cyclodextrin, etiocholan-3β-ol-17-one, M-199, DMEM, and Ham’s F-12 medium were purchased from Sigma (St. Louis, MO, United States).