1 2 dio leoyl sn glycero 3 phosphocholine

DNAs were purchased from
Eurofins Genomics
(Tokyo, Japan). Nonmodified and FAM-modified DNAs were of oligonucleotide
purification cartridge grade, and Alexa 405- and Cy3-modified DNAs
were of high-performance liquid chromatography purification grade.
DNA was stored in Ultrapure water (18 MΩ·cm in resistance)
at a concentration of 100 μM at −20 °C until use.
DNA concentration was measured using a microvolume spectrometer (DS-11FX,
DeNovix, Wilmington, DE, USA). DNA sequences are provided in Table S1. Sorbitan monooleate (Span 80) and oleylamine
were purchased from Tokyo Chemical Industry (Tokyo, Japan) and Kanto
Chemical (Tokyo, Japan), respectively. Ultrapure water and Tris-HCl
(pH 8.0) were purchased from Thermo Fisher Scientific (Waltham, MA,
USA). NaCl powder, cholesterol, polyoxyethylene (10) octylphenyl ether
(Triton-X 100), sucrose, and liquid paraffin were purchased from FUJIFILM
Wako Pure Chemical (Osaka, Japan). Iodixanol (OptiPrep) was purchased
from Cosmo Bio (Tokyo, Japan). Silicone-coated glasses (30 mm by 40
mm with a thickness of 0.17 mm) were purchased from Matsunami Glass
(Osaka, Japan). 1,2-Dioleoyl-sn-glycero-3-phosphocholine
(DOPC) was purchased from NOF Corporation (Tokyo, Japan). 1,2-Dioleoyl-3-trimethylammonium-propane
(DOTAP) was purchased from Avanti Polar Lipids (Alabaster, AL, USA).
Exonuclease I and III were purchased from New England Biolabs (Ipswich,
MS, USA).