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The H1703 is a general-purpose incubator designed for culturing cells in a controlled environment. It maintains a stable temperature, humidity, and CO2 levels to support cell growth and proliferation. The device features an easy-to-use digital interface for monitoring and adjusting the environmental conditions.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions) Nci h1299, by National Collection of Authenticated Cell Cultures (3 mentions) Sk mes 1, by National Collection of Authenticated Cell Cultures (2 mentions) Roswell park memorial institute 1640 medium, by Thermo Fisher Scientific (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) H1299, by National Collection of Authenticated Cell Cultures (1 mentions) H1975, by National Collection of Authenticated Cell Cultures (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Cell incubator, by Thermo Fisher Scientific (1 mentions) Nci h460, by National Collection of Authenticated Cell Cultures (1 mentions) H1299, by Genomed (1 mentions) Penicillin streptomycin, by Yeasen (1 mentions)

5 protocols using h1703

1

NSCLC Tissue Collection and Cell Line Culture

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Eighty-seven human NSCLC tissues and their corresponding noncancerous adjacent tissues were collected at the Affiliated Hospital of Nantong University. All of the patient materials were obtained with appropriate written informed consent, and this study was approved by the Clinical Research Ethics Committees of the Affiliated Hospital of Nantong University.
Human NSCLC cell lines, including A549, H1703, SK-MES-1, and NCI-H1299, were purchased from the Cell Bank of Type Culture Collection of Chinese Academy of Sciences (Shanghai, China). Cells were cultured in RPMI-1640 Medium (Hyclone, Beijing, China), supplemented with 10% fetal bovine serum (Hyclone) at 37°C in a 5% CO2 incubator.
Wang Q., Jiang S., Song A., Hou S., Wu Q., Qi L, & Gao X. (2017). HOXD-AS1 functions as an oncogenic ceRNA to promote NSCLC cell progression by sequestering miR-147a. OncoTargets and therapy, 10, 4753-4763.
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2

NSCLC Tissue and Cell Line Protocol

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One hundred and thirteen pairs of NSCLC tissues and corresponding noncancerous adjacent tissues were obtained from patients diagnosed with NSCLC at Department of Thoracic Surgery of The First Affiliated Hospital of Chengdu Medical College. All involved patients were informed and consent was written and collected. The study was approved by the Clinical Research Ethics Committees of The First Affiliated Hospital of Chengdu Medical College (No CDM107632). Clinical data were collected from all patients at the same time.
Human NSCLC cell lines (A549, H1703, SK-MES-1, and NCI-H1299) were purchased from the Cell Bank of Type Culture Collection of Chinese Academy of Sciences (Shanghai, People’s Republic of China). Cells were cultured in Roswell Park Memorial Institute-1640 medium (Thermo Fisher Scientific, Waltham, MA, USA) containing 10% fetal bovine serum (Thermo Fisher Scientific) and maintained at 37°C with 5% CO2.
Zhao W., Li W., Dai W., Huang N, & Qiu J. (2018). LINK-A promotes cell proliferation through the regulation of aerobic glycolysis in non-small-cell lung cancer. OncoTargets and therapy, 11, 6071-6080.
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3

NSCLC Cell Line Characterization

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Human NSCLC cell lines NCI157, A549, H1299, H460, H1703, H1975, and BEAS control cells were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). The cells were grown in RPMI-1640 medium with 10% fetal bovine serum (Gibco, United States). All the NSCLC cell lines were authenticated by short tandem repeat (STR) analysis and were tested for Mycoplasma contamination.
Han L., Lei G., Chen Z., Zhang Y., Huang C, & Chen W. (2022). IGF2BP2 Regulates MALAT1 by Serving as an N6-Methyladenosine Reader to Promote NSCLC Proliferation. Frontiers in Molecular Biosciences, 8, 780089.
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4

Cell Line Culturing Protocol

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Cell lines including H-358, H-1703, A549 and NL-20 were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China) and the American Type Culture Collection (ATCC; Rockville, MD, USA). All cells were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (both from Gibco Co., New York, NY, USA). Cell cultures were kept in cell incubators with a humidified atmosphere and 5% CO2 at 37°C.
Wang D., Cao Q., Qu M., Xiao Z., Zhang M, & Di S. (2017). MicroRNA-616 promotes the growth and metastasis of non-small cell lung cancer by targeting SOX7. Oncology Reports, 38(4), 2078-2086.
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5

Manipulation of circSWT1 and SNAIL in NSCLC

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The human normal bronchial epithelial cells (HBE) and human NSCLC cell lines (A549, NCI‐H460, PC‐9, H1703, and NCI‐H1299) were purchased from the Cell Bank of the Chinese Academy of Sciences . In DMEM and RPMI‐1640 with 10% fetal bovine serum (Gibco) and 1% penicillin–streptomycin, all the cells were grown (Yeasen). These cells were raised at 37 °C with 5% CO2 in a cell incubator (Thermo Fisher Scientific). Following the manufacturer's instructions, lentiviral vectors carrying circSWT1 and shcircSWT1 were bought from Genomeditech and transfected into A549 and H1299 cells. Genomeditech supplied the CRISPR Cas9‐gDNA technology that was utilized to eradicate the SNAIL gene from the A549 and H1299 cell lines. The sequences of shcircSWT1 are reported in Additional file 1: Table S1 and those of sgSNAIL are listed in Additional file 2: Table S2, respectively.
Long X., Wang D., Wu Z., Liao Z, & Xu J. (2022). Circular RNA hsa_circ_0004689 (circSWT1) promotes NSCLC progression via the miR‐370‐3p/SNAIL axis by inducing cell epithelial‐mesenchymal transition (EMT). Cancer Medicine, 12(7), 8289-8305.
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