Biotinylated link universal

Manufactured by Agilent Technologies

The Biotinylated Link Universal is a versatile reagent designed for use in a variety of bioanalytical applications. It serves as a universal linker, providing a means to immobilize and capture molecules of interest through its biotin-streptavidin interaction. The core function of this product is to facilitate the attachment of target analytes to a solid support for further analysis or purification.

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Lab products found in correlation

Aquatex fluid, by Merck Group (1 mentions) Strepavidin hrp, by Agilent Technologies (1 mentions) Fastdab tablets, by Merck Group (1 mentions) Power block, by BioGenex (1 mentions) Bond 3 autostainer, by Leica (1 mentions) Cytoseal xyl, by Thermo Fisher Scientific (1 mentions)

3 protocols using biotinylated link universal

Immunohistochemical Analysis of Gastric Tissue

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Samples of gastric tissue were collected from all rats, fixed in 10% neutral buffered formalin, processed conventionally, embedded in paraffin, sectioned to 4–5 μm thickness, and stained with hematoxylin and eosin or periodic acid-Schiff-alcian blue (PAS-alcian blue) for morphological evaluation and image analysis. Immunolocalization of NF-κB, iNOS, and TNF-α was studied. In brief, 3–4 μm-thick sections were incubated for 2 h with primary rabbit antibody against NF-κB, iNOS, or TNF-α (1:50 dilution; Santa Cruz Biotechnology, Santa Cruz, CA, USA). Primary antibodies were detected by biotin-labeled rabbit anti-mouse secondary antibody (Biotinylated Link Universal; DakoCytomation) followed by avidin/biotin-peroxidase detection solution (DakoCytomation). Gastric specimens were counterstained with hematoxylin for 1 min and mounted using Aquatex fluid (Merck KGaA, Germany). All specimens were incubated under the same conditions, at the same time, and with the same amount of antibodies to ensure that immunoreactivity would be comparable among the different experimental groups.

Abdelfattah M.S., Elmallah M.I., Ebrahim H.Y., Almeer R.S., Eltanany R.M, & Abdel Moneim A.E. (2019). Prodigiosins from a marine sponge-associated actinomycete attenuate HCl/ethanol-induced gastric lesion via antioxidant and anti-inflammatory mechanisms. PLoS ONE, 14(6), e0216737.

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Immunohistochemical Analysis of OSCC Biopsies

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Individual paraffin blocks of formalin-fixed OSCC biopsies were obtained from the Department of Oncology and Diagnostic Sciences, University of Maryland Dental School (Baltimore, MD) and processed for immunohistochemistry as previously described [18] (link). Briefly, tissues were deparaffinized, hydrated through graded alcohols and incubated in 3% hydrogen peroxide for 10 minutes to quench the endogenous peroxidase. Sections were then incubated in blocking solution (Power Block, BioGenex, Fremont, CA), and incubated overnight at 4°C with primary antibodies diluted in a 2% BSA/0.1% Tween 20 solution in PBS. The following antibodies were used: anti-Tiam1 (1:50 dilution, Abcam), anti-PB1 (Santa Cruz A8, 1:10 dilution). Slides were then washed in PBS, incubated with biotinylated secondary antibody (Biotinylated Link Universal, DAKO North America) for 45 minutes, and treated with strepavidin-HRP (DAKO North America) for 30 minutes at room temperature. The slides were developed in 3,3-diaminobenzidine (FASTDAB tablets; Sigma), counterstained with dilute Mayer's hematoxylin, dehydrated, and mounted. Images were taken with an Aperio ScanScope CS scanner (Aperio, Vista, CA).

Zhou H., Kann M.G., Mallory E.K., Yang Y.H., Bugshan A., Binmadi N.O, & Basile J.R. (2016). Recruitment of Tiam1 to Semaphorin 4D Activates Rac and Enhances Proliferation, Invasion, and Metastasis in Oral Squamous Cell Carcinoma. Neoplasia (New York, N.Y.), 19(2), 65-74.

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Immunohistochemical Detection of Axl Receptor

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Immunohistochemical staining of Axl (goat polyclonal, dilution 1:40, R&D Systems) was accomplished using the Bond III Autostainer (Leica Microsystems, Illinois, USA). Formalin-fixed, paraffin-embedded tissue sections were first baked and deparaffinised. Antigen retrieval was accomplished by heating the slides at 99–100°C in Bond Epitope Retrieval Solution 1 for 30 min. Sections were then incubated sequentially with endogenous peroxidase block for 5 min, primary antibody for 30 min, Biotinylated Link Universal (Dako) for 25 min, Streptavidin-HRP for 25 min, diaminobenzidine (DAB) for 10 min, and haematoxylin for 5 min. Finally, the sections were dehydrated in 100% ethanol, and mounted in Cytoseal XYL (Richard-Allan Scientific, Kalamazoo, Michigan, USA). Normal breast tissue, which was used as a positive control, shows membranous Axl staining of luminal ductal cells with variable cytoplasmic staining.

D’Alfonso T.M., Hannah J., Chen Z., Liu Y., Zhou P, & Shin S.J. (2014). Axl receptor tyrosine kinase expression in breast cancer. Journal of clinical pathology, 67(8), 690-696.

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