Twelve-month old BACHD, BE, BR, BER and WT mice (n = 7–10 per genotype) were used for indirect immunofluorescent staining for Synaptophysin, Actn2 and PSD-95 in the striatum. Antibodies against synaptophysin (mouse Alexa Fluor 488 Conjugated mAb, Millipore), Actn2 (rabbit mAb, Epitomics) and PSD-95 (mouse mAb, UC Davis/NIH NeuroMab Facility) were used following manufacturer instructions with modification. Thin coronal brain sections (5μm) containing dorsal striatum (near bregma +1.2 mm) were prepared and immunostained with antibodies against synaptophysin (1:200), Actn2 (1:500) or PSD-95 (1:500). The latter two were followed by goat-anti-rabbit or goat anti-mouse secondary antibody conjugated to Alexa Fluor 594 (1:300, Invitrogen). For each mouse, two randomly selected dorsal striatal regions (near bregma +1.2 mm) were imaged at high magnification (63x) using a Zeiss LSM510 confocal laser-scanning microscope. The indirect immunofluorescence intensities of the images were measured using Image J software (NIH). All images for different genotypes were obtained and processed under identical conditions.
Goat anti mouse secondary antibody conjugated to alexa fluor 594
Manufactured by Thermo Fisher Scientific
Goat anti-mouse secondary antibody conjugated to Alexa Fluor 594 is a laboratory reagent used to detect and visualize mouse primary antibodies in immunoassays and other applications. The Alexa Fluor 594 dye provides a red fluorescent signal when excited with appropriate light.
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2 protocols using goat anti mouse secondary antibody conjugated to alexa fluor 594
1
Synaptic Protein Expression in BACHD Mice
2
Synaptic Protein Expression in BACHD Mice
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