eicosane (melt temperature = 42 °C), a higher order alkane, is used to form the TRAPs to separate each reagent layer. First, to construct the assay, the cartridges are held vertically and filled with 50 μL solution containing 5 μM Amplex Red (Biotium, Fremont, CA, USA) and 1 mM hydrogen peroxide (Fisher Scientific). To prepare eicosane (Alfa Aesar, Haverhill, MA, USA), it is first melted by placing it in a glass vial on a hot plate at 120 °C. Furthermore, 30 μL of melted eicosane quickly hardens as it is deposited by a micropipette atop the Amplex Red layer. Then, 60 μL 0.1 M phosphate buffer is added, then another 30 μL of melted eicosane, then 60 μL 0.1 M phosphate buffer, then 30 μL of melted eicosane, then 50 μL 100 ng/mL horseradish peroxidase (HRP)-conjugated anti-rabbit IgG antibodies (ThermoFisher), then 30 μL of melted eicosane. Finally, in the top zone, 2.5 μL 40 mg/mL magnetic beads coated in SARS-CoV-2 spike RBD protein is added.
Eicosane
Manufactured by Thermo Fisher Scientific
Sourced in United States
Eicosane is a straight-chain alkane with the chemical formula C20H42. It is a colorless, waxy solid at room temperature. Eicosane is commonly used as a reference standard in analytical chemistry and as a component in various industrial applications.
Automatically generated - may contain errors
Lab products found in correlation
Hydrogen peroxide, by Thermo Fisher Scientific (1 mentions)
Diazepam d5, by Merck Group (1 mentions)
Methanol, by Thermo Fisher Scientific (1 mentions)
Chloroform, by Thermo Fisher Scientific (1 mentions)
Isopropanol, by Thermo Fisher Scientific (1 mentions)
Ethyl acetate, by Thermo Fisher Scientific (1 mentions)
Flunitrazepam, by Merck Group (1 mentions)
Temazepam, by Merck Group (1 mentions)
Crystal violet, by Merck Group (1 mentions)
Oleic acid, by Merck Group (1 mentions)
Ammonium hydroxide solution, by Thermo Fisher Scientific (1 mentions)
Sodium hydroxide pellets, by Thermo Fisher Scientific (1 mentions)
Calcium carbonate, by Merck Group (1 mentions)
Iron 2 sulphate heptahydrate, by Thermo Fisher Scientific (1 mentions)
Perfluorooctyl bromide pfob, by Fluorochem (1 mentions)
Solidspec 3700 spectrophotometer, by Shimadzu (1 mentions)
Chloroform, by Merck Group (1 mentions)
Tetracosane, by Merck Group (1 mentions)
Octadecane, by Merck Group (1 mentions)
Tricosane, by Merck Group (1 mentions)
5 protocols using eicosane
1
Eicosane-based TRAP Assay for SARS-CoV-2 Detection
2
Detecting Drug-Facilitated Sexual Assault in Beverages
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All chemicals used were analytical reagent grade or better. Chloroform, ethyl acetate, isopropanol and methanol were purchased from Fisher Chemicals; eicosane was purchased from Acros Organics; diazepam, diazepam-d5, flunitrazepam and temazepam were purchased from Sigma Chemical Company; bis(trimethyl)trifluoroacetamide (BSTFA) containing 1% trimethylchlorosilane (TMCS) derivatising agent was purchased from Supelco Analytical.
The beverages used were Bacardi Breezer, an alcopop, orange flavour, 4% by volume, Becks Beer, 5% by volume, Hardy’s Classic Selection Chardonnay 2010, 12.5% by volume, J2O apple and mango, Chekov Imperial Vodka, 37.5% by volume, and were all purchased from local supermarket stores in Cambridge, UK during the period Oct 10–July 2011. The five drinks tested in this research were selected to represent common drink types consumed by women in the 16–24 years age group – a group at considerable risk of being the victim of DFSA.
The beverages used were Bacardi Breezer, an alcopop, orange flavour, 4% by volume, Becks Beer, 5% by volume, Hardy’s Classic Selection Chardonnay 2010, 12.5% by volume, J2O apple and mango, Chekov Imperial Vodka, 37.5% by volume, and were all purchased from local supermarket stores in Cambridge, UK during the period Oct 10–July 2011. The five drinks tested in this research were selected to represent common drink types consumed by women in the 16–24 years age group – a group at considerable risk of being the victim of DFSA.
3
Nanomaterials Synthesis and Characterization
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Barium sulphate (98%), eicosane (99%), octacosane (99%) were purchased from Acros Organics. Oleic acid (technical grade, 90%), calcium carbonate (99%), agar (powder, for microbiology), iron (II, III) oxide (nanopowder, 50–100 nm particle size (SEM), 97% trace metals basis, used as a magnetite XRD standard) and Crystal Violet (hexamethylpararosaniline chloride, >90%) were purchased from Sigma-Aldrich. Iron(III) chloride hexahydrate (98%) was purchased from Alfa Aesar reagents. Iron(II) sulphate heptahydrate (98%), sodium hydroxide pellets (Analytical reagent grade) and ammonium hydroxide solution (28% in water) were purchased from Fisher Scientific, and perfluorooctyl bromide (PFOB, 97%) from Fluorochem Ltd. Iohexol (Omnipaque 300) was obtained from GE healthcare. Aspirin tablets were bought commercially from Boots Ltd. Laboratory solvents were purchased from Sigma Aldrich Limited and were of analytical grade. Aqueous solutions were prepared using UHQ deionised water with a resistivity of not less than 18.2 MΩ cm−1. N2 (oxygen free) gas was purchased from BOC Ltd. and used as received. Gelatin capsules were purchased from CapsulCN, China.
4
Rare Earth Metal Organometallic Synthesis
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All reactions and manipulations were carried out under anaerobic and anhydrous conditions in an argon filled glovebox (Vigor). All syntheses and manipulations were carried out using disposable plastic spatulas. Tetrahydrofuran (thf), hexanes, toluene and diethyl ether were dried and deoxygenated using a solvent purification system (JC Meyer Solvent Systems) and were stored over molecular sieves in an argon-filled glovebox. Anhydrous dysprosium(iii ) iodide and iodine were purchased from Alfa Aesar. Eicosane was purchased from Acros Organics. Sublimed anhydrous erbium(iii ) iodide was generously donated by Prof. Tim Hughbanks' group (Texas A&M). fc[HNSi(t-Bu)Me2]2 was prepared as previously described.39 (link) Benzyl potassium was prepared via the deprotonation of toluene by nBuLi/KOtBu as described previously.64 (link) UV-vis-NIR spectra were recorded using a Shimadzu SolidSpec-3700 spectrophotometer over a range of 300 nm to 2000 nm and matched screw-capped quartz cuvettes. Elemental analyses were carried out by Midwest Microlab (Indianapolis, IN).
5
Perfluorocarbon Lipid Nanoparticle Synthesis
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The following chemicals were used as received: perfluoropentane (C5F12, 98%, Strem Chemicals, Newburyport, MA, USA); perfluorohexane (C6F14, 99%, FluoroMed, Round Rock, TX, USA); perfluorododecane (>99%, Fluoryx Labs, Carson City, NV, USA); Krytox 157 FSH oil (Miller-Stephenson Chemicals, Danbury, CT, USA); 1,2-dibehenoyl-sn-glycero-3-phosphocholine (DBPC) (99%, Avanti Polar Lipids, Alabaster, AL, USA); N-(methylpolyoxyethylene oxycarbonyl)-1,2-distearoyl-sn-glycero-3-phosphoethanolamine (DSPE-PEG5K) (NOF America, White Plains, NY, USA); octadecane (99%), heneicosane (98%), tricosane (99%), tetracosane (99%), and chloroform (≥99.9%) (Sigma-Aldrich, St. Louis, MO, USA); nonadecane (99%, Acros Organics, NJ, USA); eicosane (99%, Alfa Aesar, Ward Hill, MA, USA); docosane (98%, TCI, Portland, OR, USA); DiO fluorescent probe (excitation, 484 nm; emission, 501 nm) (Invitrogen, Eugene, OR, USA); and ultrapure deionized (DI) water from Millipore Direct-Q (Millipore Sigma, St. Louis, MO, USA).
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