Blood from healthy pregnant women was collected from the maternal vein before delivery (n = 40) and from the umbilical cord vein immediately after delivery of the placenta (n = 40). Blood samples were collected in S-Monovette serum tubes (Sarstedt AG, Germany) and processed within 1 h. Samples were allowed to clot at RT and then serum was obtained after centrifugation at 2000 g for 10 mins at 4 °C. Serum samples were aliquoted and stored at −20 °C until further use as described in77 (link),78 (link).
S monovette serum tubes
Manufactured by Sarstedt
Sourced in Germany
The S-Monovette serum tubes are blood collection tubes used to collect and store serum samples. They are designed to facilitate the coagulation process and separate the serum from the blood cells. The S-Monovette serum tubes are made of high-quality materials and are intended for in vitro diagnostic use.
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4 protocols using s monovette serum tubes
1
Maternal-Fetal Serum Sampling Procedure
2
Hemolysis Reduction in ED Blood Samples
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This study was based on the systematic identification of hemolysis in serum samples received in the clinical laboratory of the University Hospital of Parma from the local ED, a large urban facility with approximately 92.000 visits per year. In this ED, blood samples are typically drawn using intravenous catheters (1.0 x 3.2 mm, 20-gauge catheter; Neo DELTA VEN, Viadana, MN, Italy). According to the study protocol, the investigation was divided in two observational periods of 4 months each.
In the former period (i.e., from the 1st of January to the 30th of April, 2014), blood was collected from intravenous catheters using 13x100 mm x 5.0 mL BD Vacutainer® SST II Plus plastic serum tubes (Becton Dickinson Italia S.p.A, Milan, Italy). According to the positive outcome of a previous local investigation (8 (link)), the Direction of the University Hospital of Parma authorized the complete replacement of these serum tubes with 4.0 mL S-Monovette serum tubes (S-Monovette, Sarstedt AG & Co., Nümbrecht, Germany) on May 15th, 2014. After 15 days of familiarization with the new blood collection device, the second observational period started on the 1st of June, 2014, and was concluded on the 30th of September, 2014. In this second phase of the study, blood was collected from intravenous catheters by the ED personnel always using S-Monovette blood tubes in aspiration mode.
In the former period (i.e., from the 1st of January to the 30th of April, 2014), blood was collected from intravenous catheters using 13x100 mm x 5.0 mL BD Vacutainer® SST II Plus plastic serum tubes (Becton Dickinson Italia S.p.A, Milan, Italy). According to the positive outcome of a previous local investigation (8 (link)), the Direction of the University Hospital of Parma authorized the complete replacement of these serum tubes with 4.0 mL S-Monovette serum tubes (S-Monovette, Sarstedt AG & Co., Nümbrecht, Germany) on May 15th, 2014. After 15 days of familiarization with the new blood collection device, the second observational period started on the 1st of June, 2014, and was concluded on the 30th of September, 2014. In this second phase of the study, blood was collected from intravenous catheters by the ED personnel always using S-Monovette blood tubes in aspiration mode.
3
Blood Serum Sample Preparation
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Blood samples were collected into 7.5 mL S-Monovette serum tubes (Sarstedt; Numbrecht, Germany) and placed at room temperature for two hours to allow coagulation, according to standard protocols. After this, the tubes were centrifuged at 1,100 × g for ten minutes to pellet the clotted material and other debris. The resulting serum supernatants were transferred into LoBind Eppendorf tubes (Hamburg, Germany) and stored at -80°C.
4
Blood Sample Collection and Processing
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Blood samples were taken from the median cubital vein using S-Monovette-EDTA K2 tubes (SARSTEDT AG & Co. KG, Nümbrecht, Germany) for morphology and S-Monovette serum tubes (SARSTEDT AG & Co. KG, Nümbrecht, Germany) for the other markers’ determination (Figure 2 ). The subjects were fasted before blood sampling and were not physically active for at least 48 h before blood collection. Serum samples were left to clot for 45 min before centrifugation and then centrifuged at 3000× g and +8 °C for 10 min. Aliquots of serum were stored at −80 °C.
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