Rabbit polyclonal antibody against ev71 vp1

Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS) and RPMI 1640 were purchased from Thermo Scientific HyClone (UT, USA). Hybond C membrane and ECL Western blot detection system were from Pierce (Rockford, IL, USA). Rabbit polyclonal antibodies against JNK, p-JNK, p38 MAPK, p-p38 MAPK, c-Fos, p-c-Fos, c-Jun, p-c-Jun and horseradish peroxidase (HRP) conjugated goat anti-rabbit IgG were purchased from SAB (Pearland, TX, USA). Antibodies against anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were obtained from ProteinTECH Group (Chicago, IL, USA). Rabbit polyclonal antibody against EV71 VP1 was purchased from Abcam (Cambridge, UK). The JNK1/2 and p38 MAPK specific inhibitor (SP600125 and SB203580) were acquired from LC Laboratories (Woburn, MA, USA) and freshly prepared using DMSO solution.

Polydatin (C₂₀H₂₂O₈; MW, 390.39; purity, > 95%); resveratrol (C₁₄H₁₂O₃; MW, 228.24; purity, 99%) were purchased from Aladdin Co., Ltd. (Shanghai, China). Ribavirin was provided by the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). RD cells were purchased from Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). DMSO was ordered from Borunlaite Co., Ltd. (Beijing, China). Dulbecco's modified Eagle's medium (DMEM) and fetal bovine serum (FBS) were purchased from Thermo Scientific HyClone (UT, USA). Hybond C membrane and ECL Western blot detection system were purchased from Pierce Company (Rockford, IL, USA). Rabbit polyclonal antibodies against IKKα, p-IKKα, IKKβ, p-IKKβ, IKKγ, p-IKKγ, IKBα, p-IKBα, NF-κB p50, p-NF-κB p50, NF-κB p65, p-NF-κB p65, and horseradish peroxidase (HRP) conjugated goat anti-rabbit IgG secondary antibodies were purchased from SAB Company (Pearland, TX, USA). Antibodies against anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were obtained from ProteinTECH Group (Chicago, IL, USA). Rabbit polyclonal antibody against EV71/VP1 was purchased from Abcam Company (Cambridge, UK).

Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS) and RPMI 1640 were purchased from Thermo Scientific HyClone (UT, USA). Hybond C membrane and ECL Western blot detection system were from Pierce (Rockford, IL, USA). Rabbit polyclonal antibodies against MEK1/2, p-MEK1/2, ERK1/2, p-ERK1/2, c-Fos, p-c-Fos, c-Jun, p-c-Jun, p-c-myc, c-myc, Elk1, p-Elk1, and HRP conjugated goat anti-rabbit IgG were purchased from SAB (Pearland, TX, USA). SOS1 mouse monoclonal antibody was provided from abnova Company (Taiwan). Antibodies against anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were obtained from ProteinTECH Group (Chicago, IL, USA). Rabbit polyclonal antibody against EV71/VP1 was purchased from Abcam Company (Cambridge, UK). The MEK1/2 specific inhibitor U0126 was acquired from LC Laboratories (Woburn, MA, USA) and freshly prepared as DMSO solution.