Anti lamp2 h4b4

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Anti-LAMP2 (H4B4) is a primary antibody that specifically binds to the LAMP2 protein. LAMP2 is a lysosomal membrane protein involved in the regulation of autophagy. This antibody can be used for the detection and analysis of LAMP2 expression in various cell and tissue samples.

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LAMP2 (35 citations) Anti-LAMP2 (33 citations)

4 protocols using anti lamp2 h4b4

Western Blot Analysis of Postmortem Brain Lysates

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Postmortem brain lysates were prepared from slices of individual tissues, extracts were resolved by sodium dodecylsulfate-polyacrylamide gel electrophoresis followed by Western blotting as previously described [33] . The following antibodies were employed: a mouse monoclonal anti-LAMP2 (H4B4) (1:2000, Santa Cruz Biotechnology, sc18822), a mouse monoclonal anti-LAMP1 (H4A3) (1:2000, Santa Cruz Biotechnology, sc20011), a rabbit monoclonal anti-ACTIN (1:400, Sigma-Aldrich, A2066/030M4844), a monoclonal anti-α-Tubulin (clone DM1A, 1:10,000, Sigma-Aldrich T6199), a rabbit polyclonal Anti-Phospho-AKT473 antibody (1:500, Cell Signaling, 9271), a rabbit polyclonal Anti-AKT antibody (1:500, Cell Signaling, 9272), a rabbit polyclonal Anti-Phospho-ERK antibody (1:500, Cell Signaling, 4370) or a rabbit polyclonal anti-ERK antibody (1:500, Cell Signaling, 4695). The final detection was performed as previously described [33] .

Fernández B., Ferrer I., Gil F, & Hilfiker S. (2017). Biomonitorization of iron accumulation in the substantia nigra from Lewy body disease patients. Toxicology Reports, 4, 188-193.

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Postmortem Brain Protein Analysis

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Postmortem brain lysates were prepared from slices of individual
tissues, extracts were resolved by sodium dodecylsulfate-polyacrylamide gel
electrophoresis followed by Western blotting as previously described [33 (link)]. The following antibodies were
employed: a mouse monoclonal anti-LAMP2 (H4B4) (1:2000, Santa Cruz
Biotechnology, sc18822), a mouse monoclonal anti-LAMP1 (H4A3) (1:2000, Santa
Cruz Biotechnology, sc20011), a rabbit monoclonal anti-ACTIN (1:400,
Sigma-Aldrich, A2066/030M4844), a monoclonal anti-α-Tubulin (clone DM1A,
1:10,000, Sigma-Aldrich T6199), a rabbit polyclonal Anti-Phospho-AKT473 antibody
(1:500, Cell Signaling, 9271), a rabbit polyclonal Anti-AKT antibody (1:500,
Cell Signaling, 9272), a rabbit polyclonal Anti-Phospho-ERK antibody (1:500,
Cell Signaling, 4370) or a rabbit polyclonal anti-ERK antibody (1:500, Cell
Signaling, 4695). The final detection was performed as previously described
[33 (link)].

Fernández B., Ferrer I., Gil F, & Hilfiker S. (2017). Biomonitorization of iron accumulation in the substantia nigra from Lewy body disease patients. Toxicology Reports, 4, 188-193.

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Comprehensive Western Blot Analysis

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Western blot analysis was performed as previously described^{53 (link)}. The following antibodies were used: anti-HDAC6 (sc-11420, Santa Cruz), anti-HDAC10 (H3413, Sigma), anti-acetylated histone H3 (06-911, Merck Millipore), anti-histone H3 (9715, Cell Signaling Technology, Cambridge UK), anti-LAMP-1 (H4A3, DHSB, Iowa City, IA, USA), anti-LAMP-2 (H4B4; Santa Cruz Biotechnology), anti-acetylated tubulin (6–11B-1; Sigma), anti-tubulin (2148, Cell Signaling Technology), anti P-glycoprotein (13978 S, Cell Signaling Technology) and anti-β-actin (clone AC-15; Sigma).

Ridinger J., Koeneke E., Kolbinger F.R., Koerholz K., Mahboobi S., Hellweg L., Gunkel N., Miller A.K., Peterziel H., Schmezer P., Hamacher-Brady A., Witt O, & Oehme I. (2018). Dual role of HDAC10 in lysosomal exocytosis and DNA repair promotes neuroblastoma chemoresistance. Scientific Reports, 8, 10039.

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Protein Expression Analysis by Western Blot

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Western blot analysis was performed as previously described.^{66 (link)} The following antibodies were used: anti-LC3B (L7543; Sigma), anti-p62/SQSTM1 (MBL-M162-3B), anti-HDAC6 (sc-11420, Santa Cruz), anti-HDAC10 (H3413, Sigma), anti-PARP (4C10-5; BD Pharmingen, Heidelberg, Germany), anti-Beclin-1 (D-18; Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-FOXO3A (2497 s, Cell Signaling), anti-LAMP2 (H4B4; Santa Cruz Biotechnology), anti-acetylated tubulin (6-11B-1; Sigma), anti-GAPDH (JC1682928, Millipore, Darmstadt, Germany), anti-ULK1 (#8054; Cell Signaling), anti-ATG16L2 (AP11695c-AB; Abgent), anti-MAP1LC3A (AP1805a; Abgent, San Diego, CA, USA), anti-ATG5 (#2630; Cell Signaling, Leiden, Netherlands), anti-ATG7 (#2631, Cell Signaling), anti-p-mTOR (Ser2448; Upstate), anti-p-S6K1 (Thr412; Upstate) and anti-β-actin (clone AC-15; Sigma). Ratios were calculated with the Bio-1D Version 12.10a software (Peqlab, Erlangen, Germany).

Bingel C., Koeneke E., Ridinger J., Bittmann A., Sill M., Peterziel H., Wrobel J.K., Rettig I., Milde T., Fernekorn U., Weise F., Schober A., Witt O, & Oehme I. (2017). Three-dimensional tumor cell growth stimulates autophagic flux and recapitulates chemotherapy resistance. Cell Death & Disease, 8(8), e3013-.

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