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Hexobarbital

Manufactured by Merck Group
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Hexobarbital is a barbiturate compound that is used as a laboratory reagent. It functions as a central nervous system depressant. Detailed information on its intended use is not available.

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Lab products found in correlation

Purelab classic uvf, by Elga LabWater (1 mentions) Efavirenz, by LGC (1 mentions) Whatman, by Cytiva (1 mentions) Pyrene, by Merck Group (1 mentions) Anthracene, by Merck Group (1 mentions) Caffeine, by Merck Group (1 mentions) Formic acid, by Merck Group (1 mentions) N heptane, by Avantor (1 mentions) Selegiline, by Merck Group (1 mentions) Skf525, by Merck Group (1 mentions)

3 protocols using hexobarbital

1

Quantification of Efavirenz in Breast Milk

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Reference standard of efavirenz was obtained from Toronto Research Chemicals
Inc. (North York, ON, Canada) and hexobarbital, used as an internal standard (IS),
was obtained from Sigma-Aldrich (Gillingham, Dorset, UK). LC-MS grade acetonitrile
was obtained from Fisher scientific (Loughborough, Leicestershire, UK), methanol
from VWR International (Lutterworth, Leicestershire, UK) and water was produced from
an Elga Option 4 water purifier (Elga Labwater, High Wycombe, Buckinghamshire, UK)
and was further purified to 18.2 MΩ with a Purelab Classic UVF (Elga
LabWater, High Wycombe, Buckinghamshire, UK). Whatman 903 Protein Saver cards were
obtained from Scientific Laboratory Supplies (Hessle, East Yorkshire, UK). Blank
breast milk samples were obtained (with ethics approval) from Wirral Mothers’
Milk Bank, Clatterbridge Hospital, Wirral, UK and whole blood was obtained from
drug-free healthy volunteers.
Olagunju A., Bolaji O.O., Amara A., Waitt C., Else L., Soyinka J., Adeagbo B., Adejuyigbe E., Siccardi M., Back D., Owen A, & Khoo S. (2014). Development, validation and clinical application of a novel method for the quantification of efavirenz in dried breast milk spots using LC-MS/MS. The Journal of antimicrobial chemotherapy, 70(2), 555-561.
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2

Preparation and Characterization of Chemical Solutions

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All chemicals were used as received. Caffeine, formic acid, Pirkle’s alcohol, hexobarbital, anthracene, and pyrene were purchased from Sigma-Aldrich GmbH (Taufkirchen, BY, DEU). HPLC grade chloroform (CHCl3, purity: 99.8%) and n-heptane (purity: 97%) were acquired from VWR International GmbH (Darmstadt, HE, DEU). Photoresist AZ 1518, Developer AZ 351B, and chromium etchant AZ® ECI 3000 were bought from Microchemicals GmbH (Ulm, BW, DEU). High-purity water was obtained from a Smart2Pure purifying system (0,055 μS/cm, TKA Wasseraufarbeitungssysteme GmbH, Niederelbert, RP, DEU). Starting materials and product of the model reaction N-phenyl-1,2,3,4-tetrahydroisoquinoline were provided by the Zeitler research group (Institute for Organic Chemistry, Leipzig University, Leipzig, SN, DEU) and synthesized as previously reported [68 (link)]. The following solutions were prepared: Pirkle’s alcohol, hexobarbital, pyrene in n-heptane with the concentrations 0.18 mM, 0.05 mM, 5 mM, and 5.6 mM, respectively. pyrene, anthracene, N-phenyl-1,2,3,4-tetrahydroisoquinoline, and CBrCl3 in CHCl3 have the concentrations 5 mM, 5.6 mM, 1 mM, and 1 mM respectively. Caffeine in CHCl3 have concentrations of 1.5, 3.0, 10, 20, and 50 μM. pyrene in n-heptane have concentrations of 0.05, 0.25, 0.5, and 1 mM.
Urban R.D., Fischer T.G., Charvat A., Wink K., Krafft B., Ohla S., Zeitler K., Abel B, & Belder D. (2021). On-chip mass spectrometric analysis in non-polar solvents by liquid beam infrared matrix-assisted laser dispersion/ionization. Analytical and Bioanalytical Chemistry, 413(6), 1561-1570.
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3

Pharmacological Inhibitors of Monoamine Oxidase

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Hexobarbital and Selegiline were purchased from Sigma-Aldrich, Inc. (St-Louis, MO, USA); Сlorgyline was purchased from Sigma (Basel, Switzerland); and SKF525 from Sigma (Saint Quentin Fallavier, France).
Kozochkin D.A., Manukhina E.B., Downey H.F., Tseilikman O.B., Komelkova M.V., Vasilyeva M.V., Lapshin M.S., Sahabutdinov M.N., Lazuko S.S, & Tseilikman V.E. (2017). The role of microsomal oxidation in the regulation of monoamine oxidase activity in the brain and liver of rats. General physiology and biophysics, 36(4).
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