Cells were lysed using 1X RIPA buffer containing protease and phosphatase inhibitors. Protein concentration was quantified using the BCA Protein Assay (Thermo Fischer Scientific). Equal amounts of total protein were added to a 4–12% Bis-Tris gel (Life Technologies), separated by size using electrophoresis, and transferred to a PVDF membrane. Blots were blocked in 5% skimmed milk and probed with primary antibodies against IDH1 (Invitrogen, OTI2H9) and α-tubulin (Invitrogen, 11,224-1-AP). Chemiluminescent (32,106, Thermo Fisher Scientific) signal was captured using a digital imager (Odyssey Imaging system).
Oti2h9
Manufactured by Thermo Fisher Scientific
The OTI2H9 is a laboratory equipment designed for measuring and analyzing optical transmittance. It is a compact and reliable instrument that can be used in various research and testing applications.
Automatically generated - may contain errors
Lab products found in correlation
Bis tris gel, by Thermo Fisher Scientific (2 mentions)
Bca protein assay, by Thermo Fisher Scientific (2 mentions)
Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions)
Pvdf membrane, by Thermo Fisher Scientific (1 mentions)
Chemiluminescent, by Thermo Fisher Scientific (1 mentions)
2 protocols using oti2h9
1
Western Blot Analysis of IDH1
2
Western Blot Analysis of IDH1 Protein
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were lysed using 1X ice-cold RIPA buffer supplemented with protease and phosphatase inhibitors. Protein concentration was quantified using the Pierce™ B.C.A. Protein Assay kit (Thermo Fischer Scientific). Equal amounts of whole protein extracts were loaded and separated by electrophoresis on a 4–12% Bis-Tris gel (Life Technologies) and transferred to a PVDF membrane (Thermo Fischer Scientific). Blots were blocked in 5% (wt/vol) non-fat milk and then probed with antibodies against anti-IDH1 (Invitrogen, OTI2H9) and anti-beta-actin (Invitrogen, 15739-BTIN). Chemiluminescent (32106, Thermo Fisher Scientific) signal was captured using a digital imager (Odyssey Infrared Imaging system).
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