Erβ ab3576

Manufactured by Abcam

Sourced in United States

ERβ (ab3576) is a primary antibody that targets the estrogen receptor beta (ERβ) protein. ERβ is a member of the nuclear receptor superfamily and functions as a transcriptional regulator in response to estrogen signaling.

Automatically generated - may contain errors

Lab products found in correlation

β actin, by Merck Group (1 mentions) Caspase 3 sc 7148, by Santa Cruz Biotechnology (1 mentions) Poly adp ribose polymerase parp sc 7150, by Santa Cruz Biotechnology (1 mentions) 17β estradiol e2, by Merck Group (1 mentions) Dual luciferase assay system, by Promega (1 mentions) 5 aza 2 deoxycytidine, by Merck Group (1 mentions) Genistein, by Merck Group (1 mentions) Mpp dihydrochloride, by Merck Group (1 mentions) Erb 041, by Merck Group (1 mentions) Cenpf ab 5, by Abcam (1 mentions)

Spelling variants of this product

Ab3576 (24 citations)

3 protocols using erβ ab3576

Combinatorial Treatment with Tamoxifen and Gemcitabine

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tam (T9262) was obtained from Sigma-Aldrich (St. Louis, MO, USA). Gem was obtained from Sagent Pharmaceuticals (Schaumburg, IL, USA). Antibodies used for western blot analysis were purchased from the following manufacturers: poly(ADP-ribose) polymerase (PARP) (sc-7150) and caspase-3 (sc7148) (Santa Cruz Biotechnology, Inc., Dallas, TX, USA); ERα (2512) and p70 S6 kinase (p70S6k, 2708) (Cell Signaling Technology, Beverly, MA, USA); ERβ (ab3576; Abcam, Cambridge, MA, USA); and β-actin (A3853; Sigma-Aldrich). For the in vitro studies, Tam and Gem were reconstituted just before use in methanol and sterile water, respectively, creating stock solutions that were then diluted in medium to obtain the final concentrations indicated in the figures.

TAKEUCHI H., MMEJE C.O., JINESH G.G., TAOKA R, & KAMAT A.M. (2015). Sequential gemcitabine and tamoxifen treatment enhances apoptosis and blocks transformation in bladder cancer cells. Oncology Reports, 34(5), 2738-2744.

+ Open protocol

+ Expand

Estrogen Receptor Beta Regulation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Genistein, 5-Aza-2′deoxycytidine, MPP dihydrochloride, 17 β-estradiol (E₂) and ERB-041 were purchased from Sigma Aldrich (St. Louis, MO). PHTPP (4-[2-Phenyl-5,7-bis(trifluoromethyl)pyrazolo[1,5-a]pyrimidin-3-yl]phenol) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA). ER-β (N-19) goat polyclonal, p-ER-β (serine 87) rabbit polyclonal, DNMT1 (H-300) rabbit polyclonal, DNMT3a (H-295) rabbit polyclonal and DNMT3b (N-19) goat polyclonal antibodies and a pool of five target-specific siRNAs designed to knockdown ER-β gene were purchased from Santa Cruz Biotechnology. ER-β (ab3576) and p-ER-β (serine 105) rabbit polyclonal antibodies were purchased from abcam (Cambridge, MA). Estrogen response element (ERE) reporter luciferase kit was purchased from Qiagen (Germantown, MD) and the Dual–Luciferase Assay System from Promega (Madison, WI).

Mahmoud A.M., Al-alem U., Ali M.M, & Bosland M.C. (2015). Genistein Increases Estrogen Receptor Beta Expression in Prostate Cancer via Reducing its Promoter Methylation. The Journal of steroid biochemistry and molecular biology, 152, 62-75.

+ Open protocol

+ Expand

Immunohistochemical Analysis of CENPF and ERβ

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

The detailed steps for conducting immunohistochemistry was described previously [11 (link)]. CENPF (ab5) and ERβ (ab3576) were purchased from Abcam. ERβ1 (MCA1974ST), ERβ2 (MCA2279GT) and ERβ5 (MCA4676T) were purchased from AbDSerotec. The specificity of the above antibodies was confirmed by numerous laboratories including ours [11 (link), 28 (link)]. Immunohistochemical method to analyze the optical density was calculated by Image-Pro Plus software.

Hexiao T., Yuquan B., Lecai X., Yanhong W., Li S., Weidong H., Ming X., Xuefeng Z., Gaofeng P., Li Z., Minglin Z., Zheng T., Zetian Y., Xiao Z., Yi C., Lanuti M, & Jinping Z. (2021). Knockdown of CENPF inhibits the progression of lung adenocarcinoma mediated by ERβ2/5 pathway. Aging (Albany NY), 13(2), 2604-2625.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!