Monoclonal anti srpk1

Manufactured by BD

Monoclonal anti-SRPK1 is a laboratory reagent used for the detection and study of SRPK1 protein in biological samples. SRPK1 is a serine/arginine-rich protein kinase that plays a role in the regulation of pre-mRNA splicing. The monoclonal antibody specifically binds to SRPK1 and can be used in various analytical techniques, such as Western blotting, immunoprecipitation, and immunohistochemistry, to investigate the expression and localization of SRPK1 in cells and tissues.

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Lab products found in correlation

Ecl system, by Merck Group (2 mentions) Las 4000 imaging system, by Fujifilm (2 mentions) Polyclonal anti rbm4, by Santa Cruz Biotechnology (1 mentions) Monoclonal anti flag m2, by Merck Group (1 mentions) Polyclonal anti lamin a c, by Santa Cruz Biotechnology (1 mentions) Monoclonal anti α tubulin, by Merck Group (1 mentions)

Close spelling variants of this product

Anti-SRPK1 monoclonal antibody (3 citations) Mouse monoclonal anti-SRPK1 (2 citations)

2 protocols using monoclonal anti srpk1

Immunoblot Analysis of RBM4, SRPK1, and MYC

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The immunoblot analysis was performed using an enhanced chemiluminescence (ECL) system (Millipore, Billerica, MA, USA), and results were monitored using the LAS-4000 imaging system (Fujifilm, Tokyo, Japan). The primary antibodies, including polyclonal anti-RBM4 (Santa Cruz Biotechnology, Santa Cruz, CA, USA), polyclonal anti-GAPDH (MDBio, Taipei, Taiwan), polyclonal anti-SP1 (Abcam, Taipei, Taiwan), monoclonal anti-SRPK1 (BD Transduction, San Jose, CA, USA), and monoclonal anti-MYC (MDBio) were applied in this study.

Lin Y.C., Lee Y.C., Lin Y.J, & Lin J.C. (2019). Berberine Promotes Beige Adipogenic Signatures of 3T3-L1 Cells by Regulating Post-transcriptional Events. Cells, 8(6), 632.

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Immunoblot Analysis of Phosphorylated RBM4

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The immunoblot analysis was conducted using an enhanced chemiluminescence (ECL) system (Millipore), and the images were analyzed with the LAS-4000 imaging system (Fujifilm). The primary antibodies used in this study included polyclonal anti-RBM4 and anti-pS309, which probed the total and phosphorylated RBM4, monoclonal anti-PTB (EMD Millipore), monoclonal anti-SRPK1 (BD Transduction Laboratories), monoclonal anti-FLAG M2 (Sigma-Aldrich), polyclonal anti-GAPDH (MDBio), polyclonal anti-lamin A/C (Santa Cruz Biotechnology), monoclonal anti-actin (Chemicon Millipore), and monoclonal anti-α Tubulin (EMD Millipore).

Lin J.C., Lin C.Y., Tarn W.Y, & Li F.Y. (2014). Elevated SRPK1 lessens apoptosis in breast cancer cells through RBM4-regulated splicing events. RNA, 20(10), 1621-1631.

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