C raf ps259

Manufactured by Cell Signaling Technology

Sourced in United States

C-Raf pS259 is a primary antibody that recognizes the phosphorylated form of the C-Raf protein at serine 259. C-Raf is a component of the MAPK/ERK signaling pathway and plays a role in cellular processes such as proliferation, differentiation, and survival.

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Lab products found in correlation

Cd11b, by BD (1 mentions) Glyceraldehyde 3 phosphate dehydrogenase gapdh, by Cell Signaling Technology (1 mentions) P47phox, by Cell Signaling Technology (1 mentions) C cbl c 15, by Santa Cruz Biotechnology (1 mentions) Horseradish peroxidase anti mouse, by Cell Signaling Technology (1 mentions) Py416 sfk, by Cell Signaling Technology (1 mentions) Ahr h211, by Santa Cruz Biotechnology (1 mentions) C cbl, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

C-Raf (66 citations)

2 protocols using c raf ps259

Antibody Panel for Flow Cytometry

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CD38 and CD11b for flow cytometry were from Becton Dickinson (Franklin Lakes, NJ). Lyn, Fgr, pY416-SFK, AhR, p47^phox, mTOR, c-Raf pS259, c-Raf pS621, c-Raf pS289/296/301(c-Raf pC-terminal domain), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), horseradish peroxidase anti-mouse, and anti-rabbit antibodies were from Cell Signaling (Danvers, MA). Total c-Raf was from Becton Dickinson. c-Cbl (C-15) and AhR (H211) were from Santa Cruz Biotechnology (Santa Cruz, CA).

MacDonald R.J., Bunaciu R.P., Ip V., Dai D., Tran D., Varner J.D, & Yen A. (2018). Src family kinase inhibitor bosutinib enhances retinoic acid-induced differentiation of HL-60 leukemia cells. Leukemia & lymphoma, 59(12), 2941-2951.

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Western Blot Analysis of Signaling Proteins

Cited 1 time

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Total cell lysate (25 µg protein) was resolved by SDS-PAGE analysis [30 (link)] using 12% acrylamide gels. Antibodies for western blotting were anti-: c-Raf pS259 (polyclonal), c-Raf pS289/296/301 (polyclonal), VAV1 (polyclonal), Lyn (clone C13F9), SFK pY416 (polyclonal), LCN2 (clone D4M8L), MMP9 (clone D6O3H), Smad2 pS245/250/255 (polyclonal), GAPDH (clone D16H11), HRP anti-mouse, and HRP anti-rabbit (Cell Signaling, MA, USA), c-Raf pS621 (polyclonal) (Thermo Fisher), c-Raf (clone 53) and CD38 (clone 22) (BD Pharmingen, San Jose, CA, USA), c-Cbl pY674 (clone EPR2227) (Abcam, MA, USA) and c-Cbl (polyclonal) (Santa Cruz, CA, USA). All antibodies were diluted 1:1000.

Bunaciu R.P., MacDonald R.J., Gao F., Johnson L.M., Varner J.D., Wang X., Nataraj S., Guzman M.L, & Yen A. (2017). Potential for subsets of wt-NPM1 primary AML blasts to respond to retinoic acid treatment. Oncotarget, 9(3), 4134-4149.

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