Bcl xl

Manufactured by Bioworld Technology

Sourced in United Kingdom, United States

Bcl-xL is a protein involved in the regulation of apoptosis (programmed cell death). It is a member of the Bcl-2 family of proteins and functions as an anti-apoptotic factor, preventing cell death. Bcl-xL plays a crucial role in maintaining cell survival and homeostasis.

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4 protocols using bcl xl

Western Blot Analysis of Liver Proteins

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Nuclear and cytosolic proteins were extracted from liver tissues with a protein extraction kit (KeyGen Biotech, Nanjing, China), and cells were lysed with RIPA buffer. Equal amounts of protein from each sample were separated by 10–15% SDS-PAGE (Bio-Rad, Hercules, USA). Blots were incubated overnight at 4 °C with the following primary antibodies: SIRT1 and p66shc (Abcam Ltd, Cambridge, UK); MnSOD, Bcl-xL and cleaved caspase-3 (Bioworld Technology, Inc., St Louis Park, MN, USA); and β-actin (Santa Cruz Biotechnology, Santa Cruz, CA, USA). The blots were then immunostained with secondary antibodies at 37 °C. The membranes were exposed to enhanced chemiluminescence-plus reagents (Beyotime Institute of Biotechnology). The images were captured using a BioSpectrum-410 multispectral imaging system and analyzed with Gel-Pro Analyzer (Version 5.0; Media Cybernetics, Rockville, MD, USA).

Shan W., Gao L., Zeng W., Hu Y., Wang G., Li M., Zhou J., Ma X., Tian X, & Yao J. (2015). Activation of the SIRT1/p66shc antiapoptosis pathway via carnosic acid-induced inhibition of miR-34a protects rats against nonalcoholic fatty liver disease. Cell Death & Disease, 6(7), e1833-.

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Apoptosis Pathway Protein Detection

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Anti-poly(ADP-ribose) polymerase (PARP), caspase-3, and caspase-8 antibodies were purchased from Cell Signaling Technology, Inc (Danvers, MA). Caspase-9 antibody was purchased from Enzo Life Sciences (Farmingdale, NY). B-cell lymphoma 2 (Bcl-2) antibody was purchased from Santa Cruz Biotechnology, Inc (Santa Cruz, CA). Bcl-xL, Bcl-2-associated X protein (Bax), and α-tubulin antibodies were purchased from Bioworld Technology (Louis Park, MN). β-Lapachone was chemically synthesized by KT&G Life Science (Suwon, Korea).

Kee J.Y., Han Y.H., Park J., Kim D.S., Mun J.G., Ahn K.S., Kim H.J., Um J.Y, & Hong S.H. (2016). β-Lapachone Inhibits Lung Metastasis of Colorectal Cancer by Inducing Apoptosis of CT26 Cells. Integrative Cancer Therapies, 16(4), 585-596.

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Cell Signaling Pathway Analysis

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Cells were seeded into 6-well plates and divided into the following groups: control, foretinib treatment, IR treatment and combination treatment. Cells were lysed using lysis buffer with phenylmethylsulfonyl fluoride (PMSF) and phosphatase inhibitor on ice. The cells were then centrifuged for 15min at 14000 rpm and the supernatant was collected to a new tube. Protein concentrations were identified by BCA Protein Quatification Kit (Vazyme Biotechnology). Primary antibodies were as follows: Bax (Cell Signaling Technology), Bcl-2 (Cell Signaling Technology), Bcl-xl (Bioworld Technology), Cdc2 (Santa Cruz Biotechnology), p-Cdc2 (Santa Cruz Biotechnology), Cyclin B1 (Cell Signaling Technology).

Chen G.Z., Dai W.S., Zhu H.C., Song H.M., Yang X., Wang Y.D., Min H., Lu Q., Liu S., Sun X.C, & Zeng X.N. (2017). Foretinib Enhances the Radiosensitivity in Esophageal Squamous Cell Carcinoma by Inhibiting Phosphorylation of c-Met. Journal of Cancer, 8(6), 983-992.

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Signaling Pathways Exploration in Cancer

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Anti-phospho-p38, anti-phospho-ERK, anti-phospho-JNK, PARP, caspase-3, caspase-8, and antibodies were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). Caspase-9 and ERK antibodies were obtained from Enzo Life Sciences (Farmingdale, NY, USA). Bcl-2, p38, JNK, and GAPDH antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa cruz, CA, USA). Bcl-xL and Bax antibodies were purchased from Bioworld Technology (Louis Park, MN, USA). Transwell chamber and matrigel were obtained from BD Biosciences (San Diego, CA, USA). β-Lapachone was chemically synthesized by KT&G Life Science (Suwon, Korea).

Kee J.Y., Han Y.H., Kim D.S., Mun J.G., Park S.H., So H.S., Park S.J., Park R., Um J.Y, & Hong S.H. (2017). β-Lapachone suppresses the lung metastasis of melanoma via the MAPK signaling pathway. PLoS ONE, 12(5), e0176937.

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