Lps strain o111 b4

The LPS (strain O111:B4) was purchased from Sigma (Sigma, L2630, USA). The rhein of analytical standard with its concentration ≥ 98% was purchased from Macklin (Macklin, R817293, China). The NFATc1 inhibitor NFATc1-IN-1 was purchased from MedChemExpress (MCE, HY-147369, USA). Biotinylated-Rhein (Bio-Rhein) and biotin were purchased from Bocong Biotech (Guangzhou, China). The anti-P65 (CST, 8242, USA), anti-phospho-P65(CST, 3033, USA), anti-CD68 (abcam, ab283654, USA), anti-CD86 (abcam, ab220188, USA), anti-Mannose Receptor (CD206) (abcam, ab64693, USA), anti-Trem2 (abcam, ab86491, USA), anti-EpCAM (Abclonal, A19301, China) anti-β-actin (CST, 3700, USA), and anti-histone H3 (CST, 4499, USA) antibodies were used for western immunoblotting or immunofluorescence staining in accordance with the experimental requirements.

LPS (strain O111:B4) was purchased from Sigma-Aldrich. AMTS was provided by Hubei Xinkang Pharmaceutical Chemical Co., Ltd (Tianmen China). Mouse monoclonal anti-F4/80 and anti-CD68 were purchased from Santa Cruz Biotechnology. Primary antibodies against cytoplasm inhibitor of kappa B alpha (IKBα), p-IKBα, NF-κB p65, p-p65, iNOS, COX2, JNK, phospho-JNK, p38, phospho-p38, Erk1/2, phospho-Erk1/2 were provided by Cell Signaling Technology Inc (Beverly, MA, United States). Mouse monoclonal β-actin and lamin B antibodies were purchased from Proteintech (Wuhan, China). Mouse TNF-α, IL-1β, IL-6, and MCP-1 enzyme-linked immunosorbent assay (ELISA) kits were purchased from R&D (Minneapolis, MN, United States). Myeloperoxidase (MPO) and nitric oxide (NO) commercial assay kits were bought from JianCheng Bio Co., Ltd (Nanjing, China). All other chemicals were of the highest quality commercially available.