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Rabbit polyclonal anti h3k4me2 antibody

Manufactured by Cell Signaling Technology
Sourced in United States

The Rabbit polyclonal anti-H3K4me2 antibody is a tool used to detect the presence and abundance of the histone H3 protein with a dimethylation modification at the fourth lysine residue. This antibody can be utilized in various experimental techniques, such as Western blotting, immunoprecipitation, and immunofluorescence, to study the epigenetic regulation of gene expression.

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2 protocols using rabbit polyclonal anti h3k4me2 antibody

1

Epigenetic and Signaling Pathways Analysis

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BPA and DEHP were purchased from J&K Chemical Ltd. (Shanghai, China). Mouse monoclonal anti-α-tubulin-FITC, phalloidin-TRITC (actin) and Hoechst 33342 were from Sigma (St. Louis, MO, USA). Rabbit polyclonal anti-H3K4me2 antibody, rabbit monoclonal anti-p-MAPK antibody and rabbit polyclonal LC3 antibody were from Cell Signaling Technology (Devers, MA, USA). Mouse monoclonal anti-5-methyl cytosine (5 mC) antibody was purchased from Abcam (Cambridge, UK). Annexin V-FITC/EGFP Apoptosis Detection Kits were purchased from Vazyme Biotech Co., Ltd. (Nanjing, China). Alexa Fluor 488 goat anti-rabbit, 594 goat anti-mouse antibodies were from Invitrogen (Carlsbad, CA, USA).
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2

Immunofluorescence Antibody Labeling

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A mouse monoclonal anti-α-tubulin-FITC antibody was from Sigma (St Louis, MO). Alexa Fluor 488, 594 antibodies were from Invitrogen (Carlsbad, CA). Rabbit polyclonal anti-γ-tubulin. Rabbit polyclonal anti-p-MAPK was from Cell Signaling Technology (Danvers, MA). Mouse monoclonal anti-5mC antibody and rabbit polyclonal anti-H3K9me2/3 antibody were from Abcam (Cambridge, UK). Rabbit monoclonal anti-H3K27me3 antibody and rabbit polyclonal anti-H3K4me2 antibody were from Cell Signaling Technology (Danvers, MA, USA).
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