Antibodies to XIAP, RIP-1, phosphorylated JNK, and phosphorylated H2AX were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibodies to actin, GAPDH, AIF, Smac/DIABLO, and calpain were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The calpain inhibitor PD150606 was purchased from Calbiochem (La Jolla, CA, USA), the pancaspase inhibitor z-VAD-fmk and necroptosis inhibitor necrostatin-1 (Nec-1) from Sigma-Aldrich (St. Louis, MO, USA) and the Ambion Silencer Select RIP-1 siRNA from Life Technologies. It was transfected into neuronally differentiated PC12 cells using Lipofectamine RNAiMAX Reagent (Life Technologies) as per manufacturer’s instructions. Alexafluor 488-conjugated anti-goat secondary antibody was purchased from Invitrogen (Carlsbad, CA, USA) and horseradish peroxidase-conjugated anti-rabbit and anti-mouse antibodies from Cell Signaling Technologies.
Alexafluor 488 conjugated anti goat secondary antibody
Manufactured by Thermo Fisher Scientific
Sourced in United States
Alexafluor 488-conjugated anti-goat secondary antibody is a fluorescently labeled detection reagent used in immunoassays and other applications involving the detection of goat primary antibodies. The antibody is conjugated with Alexafluor 488, a fluorescent dye that emits green fluorescence upon excitation.
Automatically generated - may contain errors
Lab products found in correlation
Phosphorylated jnk, by Cell Signaling Technology (1 mentions)
Lipofectamine rnaimax reagent, by Thermo Fisher Scientific (1 mentions)
Smac diablo, by Santa Cruz Biotechnology (1 mentions)
Z vad fmk, by Merck Group (1 mentions)
Necrostatin 1 nec 1, by Merck Group (1 mentions)
Phosphorylated h2a x, by Cell Signaling Technology (1 mentions)
Gapdh, by Santa Cruz Biotechnology (1 mentions)
Sc 20466, by Santa Cruz Biotechnology (1 mentions)
Cy3 conjugated anti mouse secondary antibody, by Jackson ImmunoResearch (1 mentions)
2 protocols using alexafluor 488 conjugated anti goat secondary antibody
1
Neuronal Cell Death Pathway Signaling
2
Quantifying Atrial Connexin Distribution and Fibrosis
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After optical mapping experiments, each left atrial preparation was divided into nine regions (superior septum, mid-septum, inferior septum, LA roof, posterior LA, LA floor, superior lateral LA, lateral LA, and inferolateral LA), and fresh-frozen for subsequent histomorphometry and immunohistochemistry analysis.
Cryostat sections (10 µm) were immunolabelled for connexin43 (Cx43) as previously described.18 (link) Sections were incubated with a mouse anti-Cx43 primary antibody (Chemicon MAB 3067) then with a Cy3-conjugated anti-mouse secondary antibody (Jackson). Connexin40 immunolabelling was performed using a goat anti-Cx40 primary antibody (Santa Cruz SC-20466) then with an Alexa Fluor 488-conjugated anti-goat secondary antibody (Invitrogen). The heterogeneity of connexin 40 distribution, the colocalization of connexin 40 and 43 at the intercalated disks, the size of en-face intercalated disks and the proportional occupation by connexin43 of these en-face disks were quantified.
Myocardial fibrosis was assessed by detecting autofluorescence at a wavelength of 488 nm.19 (link) The area of fibrosis was quantified using Fiji.20 (link)
Cryostat sections (10 µm) were immunolabelled for connexin43 (Cx43) as previously described.18 (link) Sections were incubated with a mouse anti-Cx43 primary antibody (Chemicon MAB 3067) then with a Cy3-conjugated anti-mouse secondary antibody (Jackson). Connexin40 immunolabelling was performed using a goat anti-Cx40 primary antibody (Santa Cruz SC-20466) then with an Alexa Fluor 488-conjugated anti-goat secondary antibody (Invitrogen). The heterogeneity of connexin 40 distribution, the colocalization of connexin 40 and 43 at the intercalated disks, the size of en-face intercalated disks and the proportional occupation by connexin43 of these en-face disks were quantified.
Myocardial fibrosis was assessed by detecting autofluorescence at a wavelength of 488 nm.19 (link) The area of fibrosis was quantified using Fiji.20 (link)
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