Dulbecco s modified eagle s medium f12

PC3, a grade IV adenocarcinoma prostate cell line, A549, a non-small cell lung cancer (NSCLC) cell line and HEK293T, a human embryonic kidney cell line as a non-tumor epithelial cell line, were available from the American Type Culture Collection (ATCC; Rockville, MD, USA). Cells were grown in the standard cell culture medium, Dulbecco’s Modified Eagle’s Medium-F12 (PanEco, Moscow, Russia), supplemented with 10% fetal bovine serum (FBS) (HYCLONE, Logan, UT, USA), 50 U/mL of penicillin and 50 µg/mL of streptomycin (PanEco, Moscow, Russia), 2 mM L-glutamine and 1 mM sodium pyruvate (PanEco, Moscow, Russia). Cells were incubated in a 5% CO₂ humidified incubator at 37 °C.

A549, a lung cancer cell line, and PC3, a prostate cancer cell line, were obtained from the American Type Culture Collection (ATCC; Rockville, MD, USA). Cells were maintained (5% CO₂, 37 °C) using Dulbecco’s Modified Eagle’s Medium-F12 (PanEco, Moscow, Russia), supplemented with 10% fetal bovine serum (HYCLONE, Logan, UT, USA), 2 mm L-glutamine and 1 mm sodium pyruvate (PanEco, Moscow, Russia).
Lipopolysaccharides (LPS) from Escherichia coli O111:B4 (L4391), nigericin (Ng) (N7143), Az (PHR1088) and Cf (C5793) were purchased from Sigma (St. Louis, MO, USA).