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Uv 1900 uv vis spectrophotometer

Manufactured by Shimadzu
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The UV-1900 is a UV-Vis spectrophotometer manufactured by Shimadzu. It is designed to measure the absorbance or transmittance of light in the ultraviolet and visible regions of the electromagnetic spectrum.

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Lab products found in correlation

Prism 8, by GraphPad (2 mentions) Lp980, by Edinburgh Instruments (1 mentions) 7500ce spectrometer, by Agilent Technologies (1 mentions) Sulfuric acid, by Merck Group (1 mentions) Folin ciocalteau s reagent, by Merck Group (1 mentions) Sodium carbonate na2co3, by Merck Group (1 mentions) Gallic acid, by Merck Group (1 mentions)

Spelling variants of this product

UV-Vis spectrophotometer (418 citations) UV spectrophotometer (197 citations) UV-1900 (131 citations) UV-1900 spectrophotometer (61 citations) UV-Vis 1900 spectrophotometer (3 citations) UV-1900 UV-VIS-NIR Spectrophotometer (3 citations) UV-1900 UV-vis (2 citations) UV-1900 UV–Vis double-beam spectrophotometer (2 citations)

21 protocols using uv 1900 uv vis spectrophotometer

1

Optical Transparency of CG and CGZP Films

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The light transmittance of CG and CGZP films in the range of 200–800 nm were recorded via UV 1900 UV-vis spectrophotometer (Shimadzu Corp., Tokyo, Japan). Before the test, the film samples were cut into 10 mm × 40 mm strips. Films with a greater opacity value are less transparent. The opacity values of the films were calculated by Equation (4) [35 (link)].

where T600 is the transmittance at 600 nm, and x is the thickness (mm) of the films which were measured using an electronic outside micrometer.
Zhang Q., Zhang H., Hui A., Ding J., Liu X, & Wang A. (2021). Synergistic Effect of Glycyrrhizic Acid and ZnO/Palygorskite on Improving Chitosan-Based Films and Their Potential Application in Wound Healing. Polymers, 13(22), 3878.
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2

Yeast Growth Monitoring via OD600

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Yeast growth was analyzed by the measurement of the wOD600 at the defined sampling points. Samples were centrifuged at 13,500 rpm for 5 min (1 mL), and after discarding the supernatant, the cells were suspended in 1 mL of deionized water and diluted to the absorbance read on the spectrophotometer (Shimadzu UV-1900 UV–VIS Spectrophotometer, Kyoto, Japan) at 600 nm.
Lopes A., Azevedo-Silva J., Carsanba E., Pintado M., Oliveira A.S., Ferreira C., Pereira J.O., Carvalho A.P, & Oliveira C. (2023). Peptide extract from spent yeast improves resistance of Saccharomyces cerevisiae to oxidative stress. Applied Microbiology and Biotechnology, 107(11), 3405-3417.
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3

Antioxidant Evaluation of Extracts

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The antioxidant activity of the extracts was assessed using the 2,2–Diphenyl–1–Picrylhydrazyl (DPPH) assay. In total, 100 μL of the extract was added to 3 mL of a DPPH ethanolic solution (0.03% w/v). The absorbance of the mixture was measured at 515 nm after its incubation for 20 min at room temperature. The calculated IC50 values refer to the sample concentration that is required to scavenge 50% of DPPH free radicals [63 (link)].
The spectrophotometric measurements were conducted In a SHIMADZU UV–1900, UV–VIS spectrophotometer. All measurements described above were conducted in triplicate, and the reported values of total carotenoids, total phenolics, and IC50 are the calculated average values.
Dardavila M.M., Pappou S., Savvidou M.G., Louli V., Katapodis P., Stamatis H., Magoulas K, & Voutsas E. (2023). Extraction of Bioactive Compounds from C. vulgaris Biomass Using Deep Eutectic Solvents. Molecules, 28(1), 415.
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4

Comprehensive Wine Analysis Protocol

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General parameters of wines (i.e., alcohol content, titratable acidity and pH) were analyzed according to the analytical methods recommended by the International Organization of Vine and Wine [51 ]. A METTLER TOLEDO Seven Compact pH/ion meter (Mettler Toledo, Columbus, OH, USA) was used for pH measurements. The spectrophotometric measurements (i.e., color intensity, CIELab coordinates, and phenolic index (I280)) of the wines were analyzed as previously reported [19 (link)] by using a SHIMADZU UV-1900 UV-Vis spectrophotometer (Shimadzu Latin America S.A., Montevideo, Uruguay). Turbidity measurements were performed by using a HANNA HI 88731-ISO turbidimeter (HANNA Instruments Chile, Santiago, Chile), and conductivity measurements were performed by using a HANNA HI 5321 conductometer (HANNA Instruments Chile, Santiago, Chile). The mini-contact test [52 ] was used to check the tartrate potential instability of wines.
Gil i Cortiella M., Ubeda C., Covarrubias J.I., Laurie V.F, & Peña-Neira Á. (2021). Chemical and Physical Implications of the Use of Alternative Vessels to Oak Barrels during the Production of White Wines. Molecules, 26(3), 554.
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5

Photophysical Characterization of Ru(II) Complexes

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Shimadzu UV-1900 UV-VIS Spectrophotometer was used for the collection of the steady state absorption spectra and Shimadzu 6000 Spectrofluorophotometer was used for luminescence emission spectra measurements in aqueous medium. LP980 Edinburgh Instruments laser flash photolysis system was used for the collection of the transient absorption spectra, and in the emission mode for the collection of the luminescence decay of the excited states. Singlet oxygen decay at 1270 nm was collected using Hamamatsu H10330-45 NIR detector as previously described.50 The excitation source is Q-smart (450 mJ) Nd:YAG Quantel Lasers at 355 nm.
Luminescence decay traces in the visible and infrared regions were found to follow a mono-exponential function. Singlet oxygen quantum yield, ΦΔ, was obtained by comparing the luminescence intensity of singlet oxygen at 1270 nm photosensitized by the current set of complexes with that obtained from the reference [Ru(bpy)3]2+ 37 (link) at zero time in air equilibrated D2O solution. Luminescence decay traces of ruthenium(ii) complexes at different oxygen concentrations were collected by purging the D2O solution for 20 minutes with argon and oxygen and in air equilibrated solutions.73
Akl H.N., Salah D., Abdel-Samad H.S., Abdel Aziz A.A, & Abdel-Shafi A.A. (2023). Fractional dependence of the free energy of activation on the driving force of charge transfer in the quenching of the excited states of substituted phenanthroline homoleptic ruthenium(ii) complexes in aqueous medium. RSC Advances, 13(19), 13314-13323.
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6

Antimicrobial Susceptibility of E. coli with KPC Variants

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The antimicrobial susceptibility of E. coli TOP10 strains containing plasmids pTOPO-KPC-2, pTOPO-KPC-41, and pTOPO-KPC-93, respectively, was determined by the broth microdilution method. Steady-state kinetic parameters of carbapenemase KPC-93 were measured in 100 mM sodium phosphate (pH 7.0). The initial velocity of hydrolysis was determined using a UV-1900 UV-Vis spectrophotometer (Shimadzu, Japan). The hydrolysis rate of nitrocefin was determined using a wavelength of 482 nm and an absorption coefficient (Δε) of 15,000 (M−1 · cm−1).
Wu Y., Yang X., Liu C., Zhang Y., Cheung Y.C., Wai Chi Chan E., Chen S, & Zhang R. (2022). Identification of a KPC Variant Conferring Resistance to Ceftazidime-Avibactam from ST11 Carbapenem-Resistant Klebsiella pneumoniae Strains. Microbiology Spectrum, 10(2), e02655-21.
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7

UV Spectroscopy of Lignin Solutions

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UV spectrophotometry
was conducted on a Shimadzu UV-1900 UV–vis spectrophotometer.
Spectra were recorded from 500 to 250 nm (or 200 nm) at 1.0 nm intervals
and medium speed. The reference cell was occupied by the same blank
solvent used for dilution, which was also used for recording the background
spectrum. These background spectra were double checked with a new
cuvette of blank solvent, where a measurement was only started if
the baseline-deviation was below 0.005 cm–1. Stock
solutions of 0.2–0.5 mg/mL lignin in blank solvent were made.
Stock solutions of lignin were always freshly prepared and measured
within 24 h of preparation. Furthermore, 200–600 μL of
stock solution were pipetted into the quartz cuvette and diluted with
1600–2700 μL of blank solvent. The actual volumes were
adjusted to yield an absorbance of 0.3–1.0 cm–1 at 280 nm for each measurement. Samples were run in duplicates with
at least two dilutions, yielding at least four measurement points
per sample. All concentrations were calculated with respect to the
dry ash-free sample. The absorptivity is hence given as the absorbance
divided by the dry ash-free concentration of lignin in solution.
Ruwoldt J., Tanase-Opedal M, & Syverud K. (2022). Ultraviolet Spectrophotometry of Lignin Revisited: Exploring Solvents with Low Harmfulness, Lignin Purity, Hansen Solubility Parameter, and Determination of Phenolic Hydroxyl Groups. ACS Omega, 7(50), 46371-46383.
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8

UV-Vis Spectroscopic Analysis of Nucleic Acids

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UV-Vis spectroscopy experiments were performed on a SHIMADZU UV-1900 UV-Vis spectrophotometer (Tokyo, Japan) at room temperature. The DNA or/and RNA oligonucleotide, DNA-RNA hybrid, DNA duplex samples (2 mL) were diluted to 2.0 μM in 10 mM Tris-HCl, 50 mM NaCl buffer at pH 7.0. Spectra were recorded over a wavelength range from 350 to 200 nm with a scan rate at 100 nm/min and data interval for 1 nm. A 10 mm optical path length quartz cuvette was used for UV-Vis measurement.
Li W., Yang S., Xu P., Zhang D., Tong Y., Chen L., Jia B., Li A., Lian C., Ru D., Zhang B., Liu M., Chen C., Fu W., Yuan S., Gu C., Wang L., Li W., Liang Y., Yang Z., Ren X., Wang S., Zhang X., Song Y., Xie Y., Lu H., Xu J., Wang H, & Yu W. (2022). SARS-CoV-2 RNA elements share human sequence identity and upregulate hyaluronan via NamiRNA-enhancer network. EBioMedicine, 76, 103861.
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9

Melting Temperature Analysis of Nucleic Acid Hybrids

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Melting temperatures (Tm) of self-complementary sequences were determined from the changes in absorbance at 260 nm as a function of temperature in a 10 mm path length quartz cuvette on a SHIMADZU UV-1900 UV-Vis spectrophotometer (Tokyo, Japan) equipped with a temperature control system. Solutions (2 mL) of 2 μM pre-hybridized DNA-RNA hybrid or DNA duplex in aqueous buffer (10 mM Tris-HCl, 50 mM NaCl, pH 7.5) were equilibrated at 10 °C for 5 min and then slowly ramped to 90 °C with 2 °C step at a rate of 1 °C/min. Tm values were calculated as the first derivatives of heating curves.
Li W., Yang S., Xu P., Zhang D., Tong Y., Chen L., Jia B., Li A., Lian C., Ru D., Zhang B., Liu M., Chen C., Fu W., Yuan S., Gu C., Wang L., Li W., Liang Y., Yang Z., Ren X., Wang S., Zhang X., Song Y., Xie Y., Lu H., Xu J., Wang H, & Yu W. (2022). SARS-CoV-2 RNA elements share human sequence identity and upregulate hyaluronan via NamiRNA-enhancer network. EBioMedicine, 76, 103861.
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10

Expression and Purification of TDP-43 NTD

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Gene cloning, expression and purification of TDP-43 NTD and the Cys50Ser (C50S) single-point mutant were performed as previously described [24 (link)]. The purified protein contained 77 residues and the MHHHHHHSSGVDLGTENLYFQS sequence fused to the N-terminus for a total of 99 residues. It was maintained at 1.6–3.0 mg/mL (150–270 µM) in 5 mM sodium phosphate buffer, 50 mM NaCl, 1 mM DTT, pH 7.4, −20 °C. Protein purity was checked with SDS page. Protein concentration was measured using a SHIMADZU UV-1900 UV-Vis spectrophotometer at a wavelength of 280 nm with an extinction coefficient (ε280) of 12,950 M−1 cm−1.
Moretti M., Marzi I., Cantarutti C., Vivoli Vega M., Mandaliti W., Mimmi M.C., Bemporad F., Corazza A, & Chiti F. (2022). Conversion of the Native N-Terminal Domain of TDP-43 into a Monomeric Alternative Fold with Lower Aggregation Propensity. Molecules, 27(13), 4309.
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